Bioinspired research project a first step toward intrinsically translucent tissue
Octopuses, squids and other sea creatures can perform a disappearing act by using specialized tissues in their bodies to manipulate the transmission and reflection of light, and now researchers at the University of California, Irvine have engineered human cells to have similar transparent abilities.
In a paper published today in Nature Communications, the scientists described how they drew inspiration from cephalopod skin to endow mammalian cells with tunable transparency and light-scattering characteristics.
“For millennia, people have been fascinated by transparency and invisibility, which have inspired philosophical speculation, works of science fiction, and much academic research,” said lead author Atrouli Chatterjee, a UCI doctoral student in chemical & biomolecular engineering. “Our project – which is decidedly in the realm of science – centers on designing and engineering cellular systems and tissues with controllable properties for transmitting, reflecting and absorbing light.”
Chatterjee works in the laboratory of Alon Gorodetsky, UCI associate professor of chemical & biomolecular engineering, who has a long history of exploring how cephalopods’ color-changing capabilities can be mimicked to develop unique technologies to benefit people. His team’s bioinspired research has led to breakthrough developments in infrared camouflage and other advanced materials.
For this study, the group drew inspiration from the way female Doryteuthis opalescens squids can evade predators by dynamically switching a stripe on their mantle from nearly transparent to opaque white. The researchers then borrowed some of the intercellular protein-based particles involved in this biological cloaking technique and found a way to introduce them into human cells to test whether the light-scattering powers are transferable to other animals.
This species of squid has specialized reflective cells called leucophores which can alter the how they scatter light. Within these cells are leucosomes, membrane-bound particles which are composed of proteins known as reflectins, which can produce iridescent camouflage.
In their experiments, the researchers cultured human embryonic kidney cells and genetically engineered them to express reflectin. They found that the protein would assemble into particles in the cells’ cytoplasm in a disordered arrangement. They also saw through optical microscopy and spectroscopy that the introduced reflectin-based structures caused the cells to change their scattering of light.
“We were amazed to find that the cells not only expressed reflectin but also packaged the protein in spheroidal nanostructures and distributed them throughout the cells’ bodies,” said Gorodetsky, a co-author on this study. “Through quantitative phase microscopy, we were able to determine that the protein structures had different optical characteristics when compared to the cytoplasm inside the cells; in other words, they optically behaved almost as they do in their native cephalopod leucophores.”
In another important part of the study, the team tested whether the reflectance could potentially be toggled on and off through external stimuli. They sandwiched cells in between coated glass plates and applied different concentrations of sodium chloride. Measuring the amount of light that was transmitted by the cells, they found that the ones exposed to higher sodium levels scattered more light and stood out more from the surroundings
“Our experiments showed that these effects appeared in the engineered cells but not in cells that lacked the reflectin particles, demonstrating a potential valuable method for tuning light-scattering properties in human cells,” Chatterjee said.
While invisible humans are still firmly in the realm of science fiction, Gorodetsky said his group’s research can offer some tangible benefits in the near term.
“This project showed that it’s possible to develop human cells with stimuli-responsive optical properties inspired by leucophores in celphalopods, and it shows that these amazing reflectin proteins can maintain their properties in foreign cellular environments,” he said.
He said the new knowledge also could open the possibility of using reflectins as a new type of biomolecular marker for medical and biological microscopy applications.
This project, which received support from Defense Applied Research Projects Agency and the Air Force Office of Scientific Research, also involved researchers from the University of California, San Diego and Hamamatsu Photonics in Japan.
The Latest Updates from Bing News & Google News
Go deeper with Bing News on:
- HKU develops non-destructive method of analyzing molecules in cellson June 23, 2020 at 8:34 am
A research group led by Professor Kenneth K.Y. Wong of the Department of Electrical and Electronic Engineering at the University of Hong Kong, in collaboration with Bielefeld University in Germany, ...
- Star-shaped brain cells may play a critical role in glaucomaon June 23, 2020 at 8:02 am
After a brain injury, cells that normally nourish nerves may actually kill them instead, a new study in rodents finds. This "reactive" phenomenon may be the driving factor behind neurodegenerative ...
- Cell phone giant laying off more than 100 in Louisville as part of restructuringon June 22, 2020 at 10:40 am
The cuts impacted a number of positions, including sales employees, project managers, sales coaches and trainers. However, many positions are expected to be rehired.
- Clear signs of brain injury with severe COVID-19on June 22, 2020 at 9:42 am
Certain patients who receive hospital care for coronavirus infection (COVID-19) exhibit clinical and neurochemical signs of brain injury, a new study shows. In even moderate COVID-19 cases, finding ...
- SIX1 cooperates with RUNX1 and SMAD4 in cell fate commitment of Müllerian duct epitheliumon June 22, 2020 at 4:46 am
During female mammal reproductive tract development, epithelial cells of the lower Müllerian duct are committed to become stratified squamous epithelium of the vagina and ectocer ...
Go deeper with Google Headlines on:
Go deeper with Bing News on:
- Researchers Create Human Cells with Tunable Optical Propertieson June 4, 2020 at 5:31 am
Within these cells are leucosomes, membrane-bound particles which are composed of proteins known as reflectins, which can produce iridescent camouflage. In their experiments, the scientists cultured ...
- Scientists engineer human cells with squid-like transparencyon June 3, 2020 at 4:29 am
Within these cells are leucosomes, membrane-bound particles that are composed of proteins known as reflectins, which can produce iridescent camouflage. In their experiments, the team at UCI cultured ...
- Scientists engineer human cells with squid-like transparencyon June 3, 2020 at 12:30 am
Within these cells are leucosomes, membrane-bound particles which are composed of proteins known as reflectins, which can produce iridescent camouflage. In their experiments, the researchers ...
- Squid's colour-changing proteins make human cells TRANSPARENTon June 2, 2020 at 9:01 am
Countershading is a form of camouflage used by animals where one ... Chamaeleons have two superposed thick layers of iridescent cells that have pigment and reflect light. There are tiny crystal ...
- Secret of squid invisibility used to turn human cells transparenton June 1, 2020 at 5:00 pm
Altogether, this structure is what allows the squids to scatter light, producing iridescent camouflage. For the new study, the UC Irvine team attempted to introduce that ability into human cells.