Illustration of ultrasound-induced microbubble (MB) rupture causing temporary pores in cell membranes, which enables entry of soluble molecules such as trehalose (not to scale). CREDIT: Jonathan A Kopechek
Ensuring adequate preservation of the millions of units of blood that are donated every year presents a challenge for blood banks, as blood can typically be stored for only six weeks after donation. A potential solution to the problem attempts to dry blood by using a sugar-based preservative that organisms living in some of Earth’s most extreme environments produce to weather long periods of dryness. New work in ultrasound technology looks to provide a path to inserting these sugars into human red blood cells, in an effort to help them last for years.
Researchers at the University of Louisville have demonstrated a new way to use ultrasound to create pores in blood cells, which allows the molecule trehalose to enter the cells and prevent their degradation when dried for preservation. Oscillating microscopic bubbles of inert gases with ultrasound provides the microfluidic system with the ability to increase the number of viable cells that can be rehydrated. The researchers discuss their work in this week’s Biomicrofluidics, from AIP Publishing.
The approach could lead to ways of increasing the shelf life of blood donations from weeks to the order of years. Such advances would provide a boon to those needing blood in areas where access to donations is difficult, like on the battlefield or in space.
“What’s unique about this is there are not many other studies that look at using acoustofluidics to place a molecule like this inside red blood cells,” said author Jonathan Kopechek. “It’s also interesting, because it allows us to store blood without keeping it cool.”
When it’s not helping these extremophiles survive, trehalose is a relatively cheap sugar that is so safe that it is used as a preservative for food items, like donut glaze.
The group constructed a spiral-shaped channel that exposed blood cells to trehalose while surrounded by microbubbles. They tuned ultrasonic vibrations using several parameters until the bubbles shook nanosized holes in the membranes of the blood cells, just large enough for trehalose or a closely related fluorescent molecule called fluorescein to enter and just briefly enough to maintain the integrity of the blood.
After confirming that fluorescein could enter cells on test samples, they added trehalose to a new batch of samples, dried the blood, rehydrated it, and performed tests to count how many of the blood cells were still viable after the process.
The ultrasound technique was able to preserve a significantly higher portion of cells by adding trehalose versus leaving the trehalose out.
The group looks to improve on the yield for their technique with the hopes of verifying the effectiveness of dry-preserved blood in patients.
The Latest Updates from Bing News & Google News
Go deeper with Bing News on:
- Afridi urges artists to work with Kashmir Committee to preserve Kashmiri cultureon September 5, 2020 at 5:34 am
Shehryar Khan Afridi said the Kashmir activists to work together with Kashmir Committee to help preserve, project Kashmir cultural ...
- How is nuclear energy preserving Egypt’s past & enhancing medicine's future?on September 4, 2020 at 9:12 am
The International Atomic Energy Agency was founded in 1957 with the goal of promoting the peaceful global use of nuclear energy. #InspireMiddleEast ...
- Indian Cases Surge Again; Russian Vaccine Study: Virus Updateon September 4, 2020 at 5:41 am
The coronavirus pandemic continued to spread in India, which added more than 83,000 new confirmed infections, taking total cases in the country to almost 4 million. In Italy, former Premier Silvio ...
- AbbVie and I-Mab Enter Into Global Strategic Partnership for Differentiated Immuno-oncology Therapyon September 4, 2020 at 4:21 am
NORTH CHICAGO, Ill., and SHANGHAI, Sept. 4, 2020 /PRNewswire/ -- AbbVie (NYSE: ABBV) and I-Mab (Nasdaq: IMAB) announced today that AbbVie and ...
- Get Outdoors -- Enjoy nature on grassy roadon September 3, 2020 at 9:06 am
Just nature plain and simple and for one’s enjoyment and health. Grassy Road is 1.23 miles long. From Treasure Road hang a left on Depot Run and you will intersect Grassy Road. Or, you can travel ...
Go deeper with Google Headlines on:
Go deeper with Bing News on:
- Original Johnny White's Bar on St. Peter St. has closed permanently: 'It's so heartbreaking'on September 4, 2020 at 11:00 am
When heirs of the legendary French Quarter barkeeper Johnny White sold the Bourbon Street building bearing his name this spring, they said the original Johnny White’s Bar at 733 St.
- Why the sports world has seized this moment to recognize that Black lives matteron September 4, 2020 at 10:35 am
CBS Sports will air a series of documentaries and conversations focused on the impact of race and racism in sports history. The "Portraits in Black" programming will air on CBS from 1 p.m. to 6 p.m.
- Study: Cryopreservation Associated with Loss of Quality in Donor Stem Cell Productson September 4, 2020 at 10:18 am
Cryopreservation Associated with Loss of Quality in Donor Stem Cell ProductsPR NewswireWASHINGTON, Sept. 4, 2020Freezing is recommended for preservation during COVID-19 but may cause stem cell ...
- Florida police chiefs association proposes reforms for use of forceon September 4, 2020 at 7:35 am
The Florida Police Chiefs Association has issued a report calling for steps that include “preservation of life,” largely banning chokeholds and other neck-related restraints and stepping up ...
- Brazilian indigenous leader, 89, who fights for preservation of Amazon tests positive for Covid-19on August 31, 2020 at 8:45 am
Raoni, who is nearly 90, has been a fierce critics of Brazilian President Jair Bolsonaro's calls for the economic development of Indigenous land in the Amazon rainforest.