Researchers at Western University have developed a new way to deliver the DNA-editing tool CRISPR-Cas9 into microorganisms in the lab, providing a way to efficiently launch a targeted attack on specific bacteria.
Published today in Nature Communications, this study opens up the possibility of using CRISPR to alter the makeup of the human microbiome in a way that could be personalized and specific from person to person. It also presents a potential alternative to traditional antibiotics to kill bacteria like Staphyloccous aureus (Staph A) or Escherichia coli (E. coli).
“One of the major reasons that I am excited about this work is that it has a wide range of possible real-world applications,” said Bogumil Karas, PhD, Assistant Professor at Western’s Schulich School of Medicine & Dentistry. “It has the potential for development of next generation antimicrobial agents that would be effective even for bacteria that are resistant to all known antibiotics. This technology could also be used to help ‘good’ bacteria produce compounds to treat diseases caused by protein deficiencies.”
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats and can be programmed to target specific stretches of genetic code and to edit DNA at precise locations. Researchers use CRISPR to permanently modify genes in living cells and organisms.
In this way, CRISPR can be programmed to kill bacteria, but until now there wasn’t a way to efficiently and specifically target certain bacterial strains.
“Using CRISPR to kill things isn’t a new idea because that’s what CRISPR does naturally,” said David Edgell, PhD, Professor at Schulich Medicine & Dentistry. “The problem has always been how you get CRISPR to where you want it to go. Other delivery systems could only go to a few spots, where ours can go anywhere.”
The delivery system developed at Western uses bacteria’s natural ability to replicate – called bacterial conjugation – to deliver CRISPR to specific bacteria, in order to alter its DNA and kill it.
“Specific delivery of any therapeutic agent, including CRISPR, is usually one of the biggest bottlenecks in development of new treatments. By developing this new delivery system, we created new tools that could help us in the development of more effective therapies in the near future,” said Karas.
The team says their delivery system is not only broadly applicable, but it is also more efficient than previous systems.
“We were able to show near complete transfer of the delivery vehicle to another bacterial species under conditions where they are in intimate contact – in a biofilm. This is important because biofilms are the natural state of the majority of bacteria, and being able to transfer DNA under these conditions is typically difficult, but we found a way to make it easy and efficient,” said Gregory Gloor, PhD, Professor at Schulich Medicine & Dentistry.
The Latest on: Next generation antimicrobial agents
via Google News
The Latest on: Next generation antimicrobial agents
- Next Generation Systems Market to see Huge Growth by 2025 | NetApp, Dell, Hitachion November 27, 2020 at 3:21 pm
Global Next Generation Systems Market Comprehensive Study is an expert and top to bottom investigation on the momentum condition of the worldwide Global Next Generation Systems industry with an ...
- Prime Minister joins forces with world leaders to call for increased action to tackle the growing crisis of antimicrobial resistanceon November 22, 2020 at 4:00 pm
The Honourable Prime Minister, Sheikh Hasina, has joined other world leaders to alert countries to the accelerating crisis of antimicrobial resistance (AMR). The prime minister was speaking at the ...
- PM joins as co-chair of antimicrobial resistance groupon November 22, 2020 at 4:00 pm
By BSS, Dhaka Prime Minister Sheikh Hasina has joined as co-chair of the newly launched One Health Global Leaders Group on Antimicrobial Resistance (AMR) along with other global leaders with a call ...
- World leaders join forces to fight the accelerating crisis of antimicrobial resistanceon November 20, 2020 at 9:37 am
High-level global leaders group will advocate for urgent action to combat antimicrobial resistance across all sectors and ensure the availability of important medicines for the future 20 November 2020 ...
- Next-Generation Wound Dressing Microlyte® Matrix Heals a Majority of Chronic Wounds in a First Published Prospective Clinical Studyon November 13, 2020 at 9:24 am
(Imbed), a privately held biotech company emerging as a leader in advanced wound care, announced today the results of a first prospective clinical evaluation of its next-generation wound dressing, ...
- Acer Swift 5 2020 review: Tiger Lake comes into its ownon November 9, 2020 at 6:06 am
I haven’t yet evaluated many Intel Tiger Lake systems, but I’ve seen enough to know that there are kinks to work out. So far, there hasn’t been a Tiger Lake laptop (officially reviewed or ...
- Culture-free alternatives for characterizing bloodstream infectionson November 4, 2020 at 5:22 am
For septic patients, prompt intervention with the appropriate antimicrobial is known to ... approaches utilizing next-generation sequencing (NGS) of DNA in blood have recently been developed.
- Arixa Pharmaceuticals Announces Acquisition by Pfizer’s Hospital Businesson October 22, 2020 at 6:09 am
--(BUSINESS WIRE)--Arixa Pharmaceuticals™, Inc., a company dedicated to developing next-generation oral ... to existing intravenous-only antimicrobial agents such as avibactam and aztreonam ...
- The Role of Antimicrobial Silver Nanotechnologyon October 18, 2020 at 5:00 pm
However, manufacturers are faced with identifying which antimicrobial agent will be effective against a wide range of organisms and yet will be tolerated next to healthy tissue ... critical function ...
- Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Initiativeon August 16, 2020 at 9:19 am
Programmatic goals would include conducting translational research, treating patients, and training the next generation of ME/CFS physician ... it has been observed that many of the infectious agents ...
via Bing News