Viruses, spread through mosquito bites, cause human illnesses such as dengue fever, Zika and yellow fever. A new control technique harnesses a naturally occurring bacterium called Wolbachia that blocks replication of viruses and breaks the cycle of mosquito-borne disease, according to an international team of researchers.
“Wolbachia is present in around 50 percent of all insects,” said Beth McGraw, professor and Huck Scholar in Entomology at Penn State, who did this research while at Monash University. “Interestingly it is not present in some of the major mosquito vectors (insects that transmit pathogens). After researchers put Wolbachia into mosquitoes, they found that, quite excitingly, Wolbachia effectively vaccinates mosquitoes, preventing viruses from replicating.”
Spread by Aedes aegypti mosquitoes, dengue virus affects millions of people each year. Symptoms include fever, body aches and nausea, although a more severe version, known as dengue hemorrhagic fever, can be fatal.
In the tropics and subtropics where Ae. aegypti resides, several large releases of Wolbachia are underway to test whether Wolbachia can reduce the incidence of human disease.
In a paper published recently in Virus Evolution, McGraw and her team report that dengue virus failed to evolve resistance to Wolbachia in controlled lab-based experiments. These findings show promise for the long-term efficacy of Wolbachia following field release.
“I am continually surprised by Wolbachia,” said McGraw. “I thought we would get dengue variants that would evolve resistance. Wolbachia is doing a better job than I expected at controlling virus replication in cells.”
The researchers took dengue virus and infected mosquito cells that either had Wolbachia or were free of bacteria. After five days, they collected the viruses that had been released from the cells and used them to infect fresh cells.
“Dengue takes over the machinery of the host cells, makes lots of copies of itself, and then it buds or burst out of the cell,” explained McGraw.
After nine rounds of passaging the virus through mosquito cells, the team found that the amount of virus released was stable in the Wolbachia-free cells. However, in the presence of Wolbachia, virus levels crashed — and in some cases, disappeared completely.
Dengue viruses grown with Wolbachia were also less effective at infecting mosquito cells and had reduced ability to replicate, compared to viruses grown without the bacterium.
Although this is good news for the control of dengue and other mosquito-transmitted diseases, the researchers note the study has limitations. The researchers used mosquito cells — which may not reflect what happens within the whole insect. And outside the lab, where mosquito populations are much larger, there may be more opportunities for the virus to develop resistance to Wolbachia.
“I think our study suggests that the evolution of resistance to Wolbachia in the virus is challenging,” said McGraw. “I don’t think it’s a guarantee that the virus is not going to evolve under field conditions because the natural system is much more complex. The real experiment is being done in the field right now, because Wolbachia has been released into communities in Australia, Indonesia and Brazil, among others. Monitoring in release areas will be needed to test for the emergence of resistance in the virus.”
The Latest on: Wolbachia
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The Latest on: Wolbachia
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- Horacio Frydman, PhDon May 8, 2020 at 5:00 pm
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- Singapore releases male mosquitoes in two large residential areas to fight spread of dengueon May 6, 2020 at 3:08 am
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- Project Wolbachia expands to Choa Chu Kang and Bukit Batok to fight spread of dengueon May 6, 2020 at 1:09 am
Male Wolbachia-Aedes mosquitoes will be released at 207 housing blocks in Choa Chu Kang, Keat Hong and Hong Kah North this month, the NEA announced on Wednesday (May 6). These areas were chosen ...
- Wolbachia mosquitoes to be released in Choa Chu Kang, Bukit Batok, identified as high-risk dengue areason May 5, 2020 at 5:00 pm
SINGAPORE — The National Environment Agency (NEA) will release male Wolbachia-carrying Aedes aegypti mosquitoes at 207 blocks of flats in Choa Chu Kang and Bukit Batok this month. The selected ...
- Researchers discover microbe that could control spread of malariaon May 4, 2020 at 9:19 am
A microbe found in mosquitoes that appears to block malaria could be used to control spread of the disease in humans, according to researchers in Kenya and Britain.
- Virus-blocking Wolbachia may make dengue historyon April 23, 2020 at 5:00 pm
Simmons noted that they did a "real world" experiment and allowed mosquitoes infected with Wolbachia and uninfected mosquitoes to feed on the blood of Vietnamese dengue patients, adding that their ...
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