A research team from the University of Greifswald and Helmholtz-Zentrum-Berlin (HZB) has solved the molecular structure of the important enzyme MHETase at BESSY II. MHETase was discovered in bacteria and together with a second enzyme – PETase – is able to break down the widely used plastic PET into its basic building blocks. This 3D structure already allowed the researchers to produce a MHETase variant with optimized activity in order to use it, together with PETase, for a sustainable recycling of PET.
The results have been published in the research journal Nature Communications.
Plastics are excellent materials: extremely versatile and almost eternally durable. But this is also exactly the problem, because after only about 100 years of producing plastics, plastic particles are now found everywhere – in groundwater, in the oceans, in the air, and in the food chain. Around 50 million tonnes of the industrially important polymer PET are produced every year. Just a tiny fraction of plastics is currently recycled at all by expensive and energy-consuming processes which yield either downgraded products or depend in turn on adding ‘fresh’ crude oil.
Bacteria on PET discovered in 2016
In 2016, a group of Japanese researchers has discovered a bacterium that grows on PET and partially feeds on it. They found out that his bacterium possesses two special enzymes, PETase and MHETase, which are able to digest PET plastic polymers. PETase breaks down the plastic into smaller PET building blocks, primarily MHET, and MHETase splits this into the two basic precursor building blocks of PET, terephthalic acid and ethylene glycol. Both components are very valuable for synthesising new PET without the addition of crude oil – for a closed sustainable production and recovery cycle.
In 2018: Structure of PETase solved
In April 2018, the structure of PETase was finally solved independently by several research groups, the Diamond Light Source was also involved in the experiments. However, PETase is only part of the solution. It is equally important to characterize the structure of the second enzyme, MHETase.
Now: 3D architecture of MHETase decoded
“MHETase is considerably larger than PETase and even more complex. A single MHETase molecule consists of 600 amino acids, or about 4000 atoms. MHETase has a surface that is about twice as large as the surface of PETase and hastherefore considerably more potential to optimise it for decomposition of PET“, explains biochemist and structural biologist Dr. Gert Weber from the joint Protein Crystallography research group at the Helmholtz-Zentrum Berlin and Freie Universität Berlin. During an interim professorship at the University of Greifswald, Weber there contacted the biotechnologist Prof. Uwe Bornscheuer at the Institute of Biochemistry, who was already involved with plastic-degrading enzymes. Together, they developed the idea of solving the structure of MHETase and then using this insight to optimise the enzyme for applications in PET recycling. To do this, they first had to extract the enzyme from bacterial cells and purify it. Within this collaboration, the teams have now succeeded in obtaining the complex three-dimensional architecture of MHETase at BESSY II, the synchrotron source at HZB in Berlin.
MHETase observed “in action”
“In order to see how MHETase binds to PET and decomposes it, you need a fragment of plastic that binds to MHETase but is not cleaved by it”, explains Weber. A member of Weber’s prior research team in Greifswald, Dr. Gottfried Palm, cut up a PET bottle, chemically decomposed the PET polymer and synthesised a small chemical fragment from it that binds to MHETase but can no longer be cleaved by it. From this ‘blocked’ MHETase, tiny crystals were grown for structural investigations at the HZB. “The structural investigations enabled us to watch MHETase virtually ‘at work’ and develop strategies for how to optimise this enzyme”, explains Weber.
“Thanks to the joint research group format, we have the means to offer beamtime access on the highly demanded BESSY II MX beamlines for measurements very quickly at any time”, says Dr. Manfred Weiss, who is responsible for the BESSY II MX beamlines. The three-dimensional architecture of MHETase actually displays some special features: enzymes such as MHETase bind to their target molecule first before a chemical reaction occurs. For breakdown of a molecule you need a tailor-made enzyme: “We can now exactly localise where the MHET molecule docks to MHETase and how MHET is then split into its two building blocks terephthalic acid and ethylene glycol”, says Weber.
Next steps: Increasing the efficiency
However, neither PETase nor MHETase are particularly efficient yet. ”Plastics have only been around on this scale for a few decades – even bacteria with their rapid successions of generations and rapid adaptability have not managed to develop a perfect solution through the evolutionary process of trial and error over such a short time”, explains Weber. “Thanks to the clarification of the structure of this very important enzyme, we have now also been able to plan, produce and biochemically characterise variants that show significantly higher activity than natural MHETase and are even active against another intermediate product of PET degradation, BHET,” adds Uwe Bornscheuer.
In future, Uwe Bornscheuer will work on systematically optimising the enzymes PETase and MHETase for their task – the decomposition of PET. Gert Weber plans to supplement these studies with further work on biological structures in order to systematically develop plastic-digesting enzymes for environmental applications. Access to the measuring stations and the IT infrastructure of HZB is indispensable for this.
Outlook: perfect recycling
Producing these kinds of enzymes in closed biotechnological cycles, for example, could be a way to really break down PET plastics and other polymers into their basic building blocks. This would also be the key to ideal recycling and a long-term solution to the plastic waste problem: production of plastic would be a closed cycle and no longer dependent on crude oil.
Learn more: “Molecular scissors” for plastic waste
The Latest on: Plastics recycling
via Google News
The Latest on: Plastics recycling
- Column: More efforts being made to recycle plasticson November 27, 2020 at 9:28 am
With the very positive news about multiple vaccines that are showing great promise to beat COVID-19, the American Chemistry Council along with the ...
- Germany's Audi, tech institute launch project to recycle automotive plasticson November 27, 2020 at 8:58 am
German car maker Audi and the country’s Karlsruhe Institute for Technology (KIT) have teamed up on a pilot project for the chemical recycling of automotive plastics. Under the project, the partners ...
- Researchers turn to AI-guided robots, chemical recycling to address plastic pollution crisison November 25, 2020 at 3:40 am
UB researchers are turning to AI-guided robots and chemical recycling to make discarded plastic easier to reuse.
- Global Industrial Plastic Waste Recycling Market 2020 Business Growth Rate, Manufacturing Analysis, Size, Share, Cost Structure and Forecast to 2025on November 25, 2020 at 1:53 am
Global Industrial Plastic Waste Recycling Market 2020 by Manufacturers, Regions, Type and Application, Forecast ...
- A recycling story with twists: Plastic bottle caps may be a key to cleaning the Indian River Lagoonon November 24, 2020 at 7:43 am
Researchers found bags of bottle caps can serve as a home-base for bacteria to remove excess nitrogen from the water, lessening toxic algae blooms ...
- DisrupTECH features superior plastics recycling, smart software, predictive mappingon November 24, 2020 at 7:04 am
Cutting-edge technologies ranging from more effective plastics recycling to using AI for systems monitoring were recently showcased by a select group of Los Alamos National Laboratory scientists to ...
- Plastic Recycling Market: Will the Companies be back in action in the next upcoming yearson November 24, 2020 at 2:43 am
Research Dive :The COVID-19 effect on Global Plastic Recycling Market and How Mechanical recycling process segment will experience a noticeable growth With the emergence of the COVID-19 crisis, the ...
- Municipal Plastic Waste Recycling Market 2021 Business Opportunities, Current Trends, Challenges and Forecast 2026on November 23, 2020 at 10:28 pm
The Global Municipal Plastic Waste Recycling Market Research Forecast 2021 – 2026 provides a comprehensive analysis of the market segments, including their dynamics, size, growth, regulatory ...
- Audi explores chemical recycling of automotive plasticson November 23, 2020 at 4:02 am
A joint project between automaker Audi and the industrial resource strategies think tank at Karlsruhe Institute for Technology (KIT) is aimed at finding an alternative to energy recovery as an ...
- New solvent-based recycling process could cut down on millions of tons of plastic wasteon November 20, 2020 at 11:12 am
Multilayer plastic materials are ubiquitous in food and medical supply packaging, particularly since layering polymers can give those films specific properties, like heat resistance or oxygen and ...
via Bing News