A team of Brigham and Women’s Hospital researchers have developed a way to bioprint tubular structures that better mimic native vessels and ducts in the body.
The 3-D bioprinting technique allows fine-tuning of the printed tissues’ properties, such as number of layers and ability to transport nutrients. These more complex tissues offer potentially viable replacements for damaged tissue. The team describes its new approach and results in a paper published on Aug. 23 in Advanced Materials.
“The vessels in the body are not uniform,” said Yu Shrike Zhang, PhD, senior author on the study and an associate bioengineer in BWH’s Department of Medicine. “This bioprinting method generates complex tubular structures that mimic those in the human system with higher fidelity than previous techniques.”
Many disorders damage tubular tissues: arteritis, atherosclerosis and thrombosis damage blood vessels, while urothelial tissue can suffer inflammatory lesions and deleterious congenital anomalies.
To make the 3D bioprinter’s “ink,” the researchers mixed the human cells with a hydrogel, a flexible structure composed of hydrophilic polymers. They optimized the chemistry of the hydrogel to allow the human cells to proliferate, or “seed,” throughout the mixture.
Next, they filled the cartridge of a 3D bioprinter with this bio-ink. They fitted the bioprinter with a custom nozzle that would allow them to continuously print tubular structures with up to three layers. Once the tubes were printed, the researchers demonstrated their ability to transport nutrients by perfusing fluids.
The researchers found that they could print tissues mimicking both vascular tissue and urothelial tissue. They mixed human urothelial and bladder smooth muscle cells with the hydrogel to form the urothelial tissue. To print the vascular tissue, they used a mixture of human endothelial cells, smooth muscle cells and the hydrogel.
The printed tubes had varying sizes, thicknesses and properties. According to Zhang, structural complexity of bioprinted tissue is critical to its viability as a replacement for native tissue. That’s because natural tissues are complex. For instance, blood vessels are comprised of multiple layers, which in turn are made up of various cell types.
The team plans to continue preclinical studies to optimize the bio-ink composition and 3D-printing parameters before testing for safety and effectiveness.
“We’re currently optimizing the parameters and biomaterial even further,” said Zhang. “Our goal is to create tubular structures with enough mechanical stability to sustain themselves in the body.”
The Latest on: 3-D bioprinting
via Google News
The Latest on: 3-D bioprinting
- Silk fibers improve bioink for 3-D-printed artificial tissues and organson October 8, 2020 at 5:37 am
How do you test, in early-stage research, whether a potential pharmaceutical effectively targets a human tumor, organ, or some other part of the body? How do you grow a new hand or another body part?
- New thermofluidic technology allows researchers to bioprint cells in 3Don October 1, 2020 at 4:41 am
Researchers have used heat to develop a new way to bioengineer cells to grow a certain way and bioprint 3D tissues.
- 3D Bioprinting Market Expected to Grow at CAGR 31.25% and Forecast to 2025on September 29, 2020 at 9:49 pm
D Bioprinting Market is valued at around USD 484.50 Million in 2017 and is evaluated to achieve USD 3250.60 Million ...
- Christchurch researcher 3D bioprinting body partson September 29, 2020 at 7:44 pm
A Christchurch researcher is developing technology he hopes can print human blood vessels and tissue. Biomedical engineer, Dr Khoon Lim, has been research ...
- 3D printing is making a giant leap into health. That could change everythingon September 28, 2020 at 3:15 am
Bioprinting and bioprinters could help transform medicine, getting new organs and therapies to where they're needed faster.
- Global 3D Bioprinting Market To Flourish At Double Digit CAGR During 2020–2026 - Zion Market Researchon September 25, 2020 at 5:35 am
Zion Market Research Reports added Latest Research Report titled Global 3D Bioprinting Market To Flourish At Double Digit CAGR During 2020 2026 Zion Market Research to its Large Report database This ...
- 3-D bioprinting constructs for cartilage regenerationon September 23, 2020 at 6:33 am
They constructed the scaffolds using one-step 3-D bioprinting to provide structural support and sustained release of cells. The experiment facilitated biomimetic regeneration of the native ...
- 3-D Printing inside the Body Could Patch Stomach Ulcerson September 22, 2020 at 4:21 am
Stomach ulcers and other gastric wounds afflict one in eight people worldwide, but common conventional therapies have drawbacks. Now scientists aim to treat such problems by exploring a new frontier ...
- 3D Bioprinting Market Worth $4735.96 Million, Globally, by 2027 at 26.43% CAGR: Verified Market Researchon September 10, 2020 at 4:40 am
The global 3D bioprinting market is expected to witness significant growth during the forecast period due to the increasing adoption of 3D bioprinting in the healthcare, pharmaceutical ...
- Oxygen-releasing bioink for 3-D bioprintingon September 8, 2020 at 5:00 pm
One of the emerging approaches to building tissues is through 3-D Printing, where cells and materials can be combined to make inks that can generate tissue structures. One of the limitations for ...
via Bing News