Researchers at the Stowers Institute for Medical Research have captured the one cell that is capable of regenerating an entire organism. For over a century, scientists have witnessed the effects of this cellular marvel, which enables creatures such as the planarian flatworm to perform death-defying feats like regrowing a severed head. But until recently, they lacked the tools necessary to target and track this cell, so they could watch it in action and discover its secrets.
Now, by pioneering a technique that combines genomics, single-cell analysis, flow cytometry and imaging, scientists have isolated this amazing regenerative cell – a subtype of the long-studied adult pluripotent stem cell – before it performs its remarkable act. The findings, published in the June 14, 2018, issue of the journal Cell, will likely propel biological studies on highly regenerative organisms like planarians and also inform regenerative medicine efforts for other organisms like humans that have less regenerative capacity.
“This is the first time that an adult pluripotent stem cell has been isolated prospectively,” says Alejandro Sánchez Alvarado, Ph.D., an investigator at the Stowers Institute and Howard Hughes Medical Institute and senior author of the study. “Our finding essentially says that this is no longer an abstraction, that there truly is a cellular entity that can restore regenerative capacities to animals that have lost it and that such entity can now be purified alive and studied in detail.”
Every multicellular organism is built from a single cell, which divides into two identical cells, then four, and so on. Each of these cells contains the exact same twisted strands of DNA, and is considered pluripotent – meaning it can give rise to all possible cell types in the body. But somewhere along the way, those starter cells – known as embryonic stem cells – resign themselves to a different fate and become skin cells, heart cells, muscle cells, or another cell type. In humans, no known pluripotent stem cells remain after birth. In planarians, they stick around into adulthood, where they become known as adult pluripotent stem cells or neoblasts. Scientists believe these neoblasts hold the secret to regeneration.
Though neoblasts have been the subject of scientific inquiry since the late 1800’s, only in the last couple of decades have scientists been able to characterize this powerful cell population using functional assays and molecular techniques. Their efforts showed that this seemingly homogenous cell population was actually a conglomeration of different subtypes, with different properties and different patterns of gene expression.
“We might have to transplant over a hundred individual cells into as many worms to find one that is truly pluripotent and can regenerate the organism,” says Sánchez Alvarado. “That’s a lot of work, just to find the one cell that fits the functional definition of a true neoblast. And if we want to define it molecularly by identifying the genes that cell is expressing, we have to destroy the cell for processing. There was no way to do that and keep the cell alive to track it during regeneration.”
Sánchez Alvarado and his team began searching for a distinguishing characteristic that could identify this elusive cell ahead of time. One feature that had long been used to distinguish neoblasts from other cells is a stem cell marker known as piwi-1, so Postdoctoral Research Associate An Zeng, Ph.D., decided to start there. First, he separated the cells that expressed this marker from those that did not. Then he noticed the cells could be separated into two groups – one that expressed high levels of piwi (aptly called piwi-high) and another that expressed low levels of piwi (called piwi-low). When Zeng studied the members of these two groups, he found only those that were piwi-high fit the molecular definition of neoblasts. So he discarded the rest.
“This kind of simultaneous quantitative analysis of gene expression and protein levels had never been done before in planarians,” says Sánchez Alvarado. “We could not have done it without the amazing scientific support facilities here at Stowers, including molecular biology, flow cytometry, bioinformatics, and imaging groups. Many researchers had assumed that all cells expressing piwi-1 were true neoblasts, and it didn’t matter how much of the marker they expressed. We showed it did matter.”
Next, Zeng selected 8,000 or so of the piwi-high cells and analyzed their gene expression patterns. To his surprise, the cells fell not into just one or two, but 12 different subgroups. Through a process of elimination, Zeng excluded any subgroups with genetic signatures indicating that the cells were destined for a particular fate, like muscle or skin. That left him with two subgroups that could still be pluripotent, which he named Nb1 and Nb2.
Conveniently, the cells in subgroup Nb2 expressed a gene coding for a member of the tetraspanin protein family, a group of evolutionarily ancient and poorly understood proteins that sit on the surface of cells. Zeng made an antibody that could latch onto this protein, pulling the cells that carried it out of a mixture of other suspected neoblasts. He then transplanted the single purified cell into a planarian that had been subjected to lethal levels of radiation. Not only did these cells repopulate and rescue the irradiated animals, but they did so 14 times more consistently than cells purified by older methods.
“We have enriched for a pluripotent stem cell population, which opens the door to a number of experiments that were not possible before,” says Sánchez Alvarado. “The fact that the marker we discovered is expressed not only in planarians but also in humans suggests that there are some conserved mechanisms that we can exploit. I expect those first principles will be broadly applicable to any organism that ever relied on stem cells to become what they are today. And that essentially is everybody.”
Lay Summary of Findings
The amazing freshwater flatworm known as planaria is a favorite of scientists who study regeneration in research organisms in the hopes of unlocking this property in humans. Over a century ago, they traced planaria’s regenerative powers to a special population of adult stem cells called neoblasts. But until recently, they lacked the tools necessary to hone in further on the individual cells truly capable of regeneration. In the June 14, 2018, issue of the journal Cell, researchers from the Stowers Institute for Medical Research published a study that combined genomics, single-cell analysis, and imaging to isolate this elusive cell. Postdoctoral Research Associate An Zeng, Ph.D., his advisor Alejandro Sánchez Alvarado, Ph.D., and their Stowers collaborators report that a molecule called TSPAN-1 that sits of the surface of cells can be used to purify regenerative neoblasts from similar cell types. These findings have important implications for advancing the study of stem cell biology and regenerative medicine.
The Latest on: Regenerative medicine
via Google News
The Latest on: Regenerative medicine
- Mesoblast sees stock tumble 31% ahead of FDA adcom meetingon August 11, 2020 at 2:28 pm
Australian regenerative medicine company Mesoblast Ltd. saw its stock tumble more than 30% following the release of briefing documents from the FDA ahead of an Aug. 13 advisory committee meeting to ...
- Passion Helps Drive Japan’s SMEs To Medical Breakthroughson August 11, 2020 at 1:48 pm
It would be understandable if you think the “kenzan method” is a new form of ikebana flower arranging, but you would be wrong – by a considerable distance.
- Therapeutic Solutions International Launches Campbell Neurosciences Division aimed at Biologically Identifying and Repairing Suicide-Prone Brainson August 11, 2020 at 10:34 am
Therapeutics Solution International, Inc., (OTC Markets: TSOI), announced today formation of Campbell Neurosciences, a new division of the Company dedicated to approaching suicide as a biological ...
- Researchers create heart cells from stem cells using 3-D printingon August 11, 2020 at 9:37 am
All humans start out from a single cell which then divides to eventually form the embryo. Depending on the signals sent by their adjacent cells, these divided cells are then developed or ...
- BioStem Life Sciences Signs Manufacturing Agreement for Amniotic Membrane Allograft Platformon August 11, 2020 at 6:00 am
(GLOBE NEWSWIRE) -- BioStem Technologies, Inc. (OTC PINK: BSEM) a leading life sciences company specializing in the development and sale of perinatal tissue-based allografts for use in regenerative ...
- Securing Future Of Regenerative Medicine In Victoriaon August 10, 2020 at 12:26 am
The Victorian Government is growing the state's world-leading medical research sector, securing the retention of the national headquarters of the ...
- Why Collaboration Is Key To Success For 3D Bioprinting And Regenerative Medicineon August 7, 2020 at 8:38 am
There are several reasons why collaboration will be pivotal to the advancement of this field. First, the body of evidence for regenerative medicine tools such as 3D bioprinting needs to be further ...
- Regenerative Medicine Market Research Report – Global Forecast till 2025on August 6, 2020 at 9:48 pm
Regenerative Medicine Market AnalysisAccording to Verified Market Research, the Global Regenerative Medicine Market ...
- New Report: Regenerative Medicine & Advanced Therapies Sector Thriving Despite COVID-19on August 6, 2020 at 6:15 am
Based Therapy Developers Poised to Break Year-Over-Year Global Financing RecordsWASHINGTON, D.C., Aug. 06, 2020 (GLOBE NEWSWIRE) -- via NEWMEDIAWIRE --The Alliance for Regenerative Medicine (ARM), the ...
via Bing News