Osaka University researchers refine method of making bio-ink droplets stick to each other, enabling 3D printing of highly complex biological structures with a wide variety of cell types using inkjet printers
Printed replacement human body parts might seem like science fiction, but this technology is rapidly becoming a reality with the potential to greatly contribute to regenerative medicine. Before any real applications, “bioprinting” still faces many technical challenges. Processing the bio-ink and making it stick to itself and hold the desired printed gel structure have been proving particularly difficult especially in inkjet printing. Few methods currently exist for gluing bio-ink droplets together and these do not work for every kind of cell, motivating new alternative approaches.
Building on their previous work, researchers at Osaka University have now refined an enzyme-driven approach to sticking biological ink droplets together, enabling complex biological structures to be printed. They recently published their findings in Macromolecular Rapid Communications.
Lead author, Shinji Sakai says, “Printing any kind of tissue structure is a complex process. The bio-ink must have low enough viscosity to flow through the inkjet printer, but also needs to rapidly form a highly viscose gel-like structure when printed. Our new approach meets these requirements while avoiding sodium alginate. In fact, the polymer we used offers excellent potential for tailoring the scaffold material for specific purposes.”
Currently, sodium alginate is the main gelling agent used for inkjet bioprinting, but has some compatibility problems with certain cell types. The researchers’ new approach is based on hydrogelation mediated by an enzyme, horseradish peroxidase, which can create cross-links between phenyl groups of an added polymer in the presence of the oxidant hydrogen peroxide.
Although hydrogen peroxide itself can also damage cells, the researchers carefully tuned the delivery of cells and hydrogen peroxide in separate droplets to limit their contact and keep the cells alive. More than 90% of the cells were viable in biological test gels prepared in this way. A number of complex test structures could also be grown from different types of cells.
“Advances in induced pluripotent stem cell technologies have made it possible for us to induce stem cells to differentiate in many different ways,” co-author Makoto Nakamura says. “Now we need new scaffolds so we can print and support these cells to move closer to achieving full 3D printing of functional tissues. Our new approach is highly versatile and should help all groups working to this goal.”
Learn more: Growing organs a few ink drops at a time
The Latest on: Printed replacement human body parts
- Engineers 3-D print flexible mesh for ankle and knee braces on June 19, 2019 at 8:09 am
However, these devices are typically designed to replace or support bones and other rigid parts of the body, and are often printed from solid ... make all sorts of devices that interface with the ... […]
- Titanium Is The Perfect Metal To Make Replacement Human Body Parts on May 27, 2019 at 12:30 pm
Particularly exciting is the perfect marriage between titanium and 3D printing ... the body compared with their alternatives. Titanium alloys commonly used in load-bearing implants are significantly ... […]
- Titanium is the perfect metal to make replacement human body parts on May 26, 2019 at 1:54 pm
Today’s it’s titanium, a metal known for its strength and lightness so it’s ideal for making replacement hips, knees and other parts of our bodies ... the perfect marriage between titanium and 3D ... […]
- Here’s How 3D Printers Are Making Human Body Parts on August 22, 2018 at 7:48 am
In 2015, more than 125,000 organs, mostly kidneys, were transplanted from one human being ... is to print them. Lots of things are made these days using three-dimensional printing, and there seems to ... […]
- Scientists are 3D printing human body parts – and are nearly ready to transplant organs on June 17, 2018 at 10:50 pm
The so-called "bioprinters" use human cells as ink to create body parts These custom machines ... To fabricate an ear, the printer starts by laying down a pliable scaffold made of hydrogel (a kind of ... […]
- 3-D Organ Printing Factory Will Produce Replica Human Body Parts So Surgeons Can Practice on June 10, 2018 at 7:14 am
It's a method that can cut down planning time and potentially reduce risk, and within the next year the United States will get its first ever 3-D human organ printing factory. Paris-based medical ... […]
- Scientists Use 3-D Printers to Make Body Parts on February 19, 2018 at 11:02 pm
This story appears in the March 2018 issue of National Geographic magazine ... special 3-D printers to make living body parts. Called bioprinters, these machines use human cells as “ink.” A standard 3 ... […]
- The firm that can 3D print human body parts on November 14, 2017 at 4:09 pm
Erik Gatenholm grins widely as he presses the start button on a 3D printer, instructing it to print a life-size human nose. It sparks a frenzied 30-minute burst of energy from the printer, as its thin ... […]
- Next in 3D Printing: Human Body Parts on September 13, 2017 at 5:00 pm
Used for everything from printing tools to car parts, additive manufacturing is now being tapped for printing human body parts. At the Wake Forest Institute for Regenerative Medicine in North Carolina ... […]
- Doctors 3D-print 'living' body parts on February 15, 2016 at 6:48 pm
Custom-made, living body parts have been ... of using living tissues to repair the body. Experts described the technology, developed in the US, as a "goose that really does lay golden eggs". The idea ... […]
via Google News and Bing News