Osaka University researchers refine method of making bio-ink droplets stick to each other, enabling 3D printing of highly complex biological structures with a wide variety of cell types using inkjet printers
Printed replacement human body parts might seem like science fiction, but this technology is rapidly becoming a reality with the potential to greatly contribute to regenerative medicine. Before any real applications, “bioprinting” still faces many technical challenges. Processing the bio-ink and making it stick to itself and hold the desired printed gel structure have been proving particularly difficult especially in inkjet printing. Few methods currently exist for gluing bio-ink droplets together and these do not work for every kind of cell, motivating new alternative approaches.
Building on their previous work, researchers at Osaka University have now refined an enzyme-driven approach to sticking biological ink droplets together, enabling complex biological structures to be printed. They recently published their findings in Macromolecular Rapid Communications.
Lead author, Shinji Sakai says, “Printing any kind of tissue structure is a complex process. The bio-ink must have low enough viscosity to flow through the inkjet printer, but also needs to rapidly form a highly viscose gel-like structure when printed. Our new approach meets these requirements while avoiding sodium alginate. In fact, the polymer we used offers excellent potential for tailoring the scaffold material for specific purposes.”
Currently, sodium alginate is the main gelling agent used for inkjet bioprinting, but has some compatibility problems with certain cell types. The researchers’ new approach is based on hydrogelation mediated by an enzyme, horseradish peroxidase, which can create cross-links between phenyl groups of an added polymer in the presence of the oxidant hydrogen peroxide.
Although hydrogen peroxide itself can also damage cells, the researchers carefully tuned the delivery of cells and hydrogen peroxide in separate droplets to limit their contact and keep the cells alive. More than 90% of the cells were viable in biological test gels prepared in this way. A number of complex test structures could also be grown from different types of cells.
“Advances in induced pluripotent stem cell technologies have made it possible for us to induce stem cells to differentiate in many different ways,” co-author Makoto Nakamura says. “Now we need new scaffolds so we can print and support these cells to move closer to achieving full 3D printing of functional tissues. Our new approach is highly versatile and should help all groups working to this goal.”
Learn more: Growing organs a few ink drops at a time
The Latest on: Printed replacement human body parts
- Body unearthed from Warren County cemetery grave renews call for state actionon June 25, 2020 at 1:26 pm
The Warren County Prosecutor's Office found no criminal act occurred, but it is renewing the call for state oversight at the cemetery.
- SCRS: Automated auto body repair ‘Blueprint Optimization Tool’ estimate software now availableon June 25, 2020 at 11:37 am
The Society of Collision Repair Specialists on Thursday announced collision repairers can now buy its Blueprint Optimization Tool, an award-winning piece of software which ...
- Healthy new tissue can be 'printed' using innovative techniqueon June 25, 2020 at 5:34 am
New muscle has successfully been created in mice using a minimally invasive technique dubbed "intravital 3-D bioprinting" by a team involving UCL scientists.
- New bio-ink could be used to 3D-print body parts inside the patienton June 16, 2020 at 10:54 am
Although we're hearing more about the prospect of 3D-printed replacement body parts, those parts need to be implanted via relatively large incisions. That may not always be the case, however, thanks ...
- A new technology to print 3D tissues directly in the bodyon June 15, 2020 at 8:16 pm
In the TV series Westworld, human body parts are built on robotic frames using 3D printers. While still far from this scenario, 3D printers are being increasingly used in medicine.
- Directly printing 3-D tissues within the bodyon June 15, 2020 at 1:23 pm
In the TV series Westworld, human body parts are built on robotic frames using 3-D printers. While still far from this scenario, 3-D printers are being increasingly used in medicine. For example, 3-D ...
- Terasaki Institute: Directly Printing 3D Tissues Within the Bodyon June 15, 2020 at 1:04 pm
In the TV series Westworld, human body parts are built on robotic frames using 3D printers. While still far from this scenario, 3D printers are being increasingly used in medicine. For example, 3D ...
- Embryo-like model created from stem cells provides a 'blueprint' of the human body and could reveal the causes of birth defects and diseaseson June 10, 2020 at 7:02 pm
The development by researchers from the University of Cambridge will allow experts to study the so-called 'black box' period of human development.
- Human-like ears 3D-printed inside mice as surgery-free spare partson June 5, 2020 at 9:45 am
Human-like ears grown on the backs of mice by 3D printing under their skin show that it might one day be possible to replace body parts without the need for surgery ...
- Humans Have a ‘Salamander-Like’ Ability to Regenerate Damaged Body Parts, Study Findson October 10, 2019 at 1:16 am
Our bodies have retained the capacity to repair ... human limb regeneration,” the authors wrote in the paper. The adorable and enigmatic axolotl is capable of regenerating many different body ...
via Google News and Bing News