Researchers at Indiana University School of Medicine have successfully developed a method to grow inner ear tissue from human stem cells—a finding that could lead to new platforms to model disease and new therapies for the treatment of hearing and balance disorders.
“The inner ear is only one of few organs with which biopsy is not performed and because of this, human inner ear tissues are scarce for research purposes,” said Eri Hashino, PhD, Ruth C. Holton Professor of Otolaryngology at IU School of Medicine. “Dish-grown human inner ear tissues offer unprecedented opportunities to develop and test new therapies for various inner ear disorders.”
The study, published online May 1, 2017, in Nature Biotechnology, was led by Karl R. Koehler, PhD, assistant professor in the Department of Otolaryngology and Head and Neck Surgery at IU School of Medicine, and Dr. Hashino in collaboration with Jeffrey Holt, PhD, professor of otology and laryngology at Harvard Medical School and Boston Children’s Hospital. The research builds on the team’s previous work with a technique called three-dimensional culture, which involves incubating stem cells in a floating ball-shaped aggregate, unlike traditional cell culture in which cells grow in a flat layer on the surface of a culture dish. This allows for more complex interactions between cells, and creates an environment that is closer to what occurs in the body during development, Dr. Koehler said.
By culturing human stem cells in this manner and treating them with specific signaling molecules, the investigators were able to guide cells through key processes involved in the development of the human inner ear. This resulted in what the scientists have termed inner ear “organoids,” or three-dimensional structures containing sensory cells and supporting cells found in the inner ear.
“This is essentially a recipe for how to make human inner ears from stem cells,” said Dr. Koehler, lead author of the study and whose research lab works on modeling human development. “After tweaking our recipe for about a year, we were shocked to discover that we could make multiple inner ear organoids in each pea-sized cell aggregate.”
The researchers used CRISPR gene editing technology to engineer stem cells that produced fluorescently labeled inner ear sensory cells. Targeting the labeled cells for analysis, they revealed that their organoids contained a population of sensory cells that have the same functional signature as cells that detect gravity and motion in the human inner ear.
“We also found neurons, like those that transmit signals from the ear to the brain, forming connections with sensory cells,” Dr. Koehler said. “This is an exciting feature of these organoids because both cell types are critcal for proper hearing and balance.”
Dr. Hashino said these findings are “a real game changer, because up until now, potential drugs or therapies have been tested on animal cells, which often behave differently from human cells.”
The researchers are currently using the human inner ear organoids to study how genes known to cause deafness interrupt normal development of the inner ear and plan to start the first-ever drug screening using human inner ear organoids.
“We hope to discover new drugs capable of helping regenerate the sound-sending hair cells in the inner ear of those who have severe hearing problems,” Dr. Hashino said.
The Latest on: Human inner ear organoids
- Can organoids, derived from stem cells, be used in disease treatments?on October 26, 2019 at 5:02 pm
Organoids of the brain, small intestine, kidney, heart, stomach, eyes, liver, pancreas, prostate, salivary glands, and inner ear to name a few have already ... While scientists have been increasingly ...
- Building inner ear organoids from human pluripotent stem cells through directed self-assemblyon June 5, 2019 at 8:00 am
He then spearheaded an effort to decode the signaling mechanisms required to coax human pluripotent stem cells to become inner ear organoids. This work was recently published in Nature Biotechnology ...
- Human hair cells from a test tubeon October 2, 2018 at 6:50 am
Researchers from the University of Bern and Bern University Hospital have managed for the first time to differentiate human inner ear cells in a laboratory from ... also known as organoids. "The ...
- Generating inner ear organoids containing putative cochlear hair cells from human pluripotent stem cellson September 11, 2018 at 3:24 am
In view of the prevalence of sensorineural hearing defects in an ageing population, the development of protocols to generate cochlear hair cells and their associated sensory neurons as tools to ...
- Guest Post: Sentient Brains in a Dish? Potential Glimpses of Sentience Will Be Detectable in Future Cerebral Organoidson March 7, 2018 at 2:35 am
Recently, researchers have managed to produce human organoids which have structural and functional properties very similar to different organs, such as the retina, the intestines, the kidneys, the ...
via Google News and Bing News