Plant, yeast, even mammalian cells could be engineered into living detectors of virtually any molecule of interest to improve environmental monitoring, metabolic production of pharmaceuticals, and more
Synthetically engineered biosensors, which can be designed to detect and signal the presence of specific small molecule compounds, have already unlocked many potential applications by harnessing bacterial cells such as E. coli to sense toxins or enable bioproduction of valuable commodities including fuel, plastics, and pharmaceuticals. As of yet, however, scientists have been challenged to leverage biosensors for use in eukaryotic cells — which comprise yeast, plants and animals — because strategies-to-date are limited in the molecules they can detect and the signals they can produce.
But now, a team of researchers at the Wyss Institute for Biologically Inspired Engineering at Harvard University and Harvard Medical School (HMS) led by George Church, Ph.D., has developed a new method for engineering a broad range of biosensors to detect and signal virtually any desired molecule using living eukaryotic cells. Church, who is a Wyss Core Faculty member and the Robert Winthrop Professor of Genetics at HMS, and his team reported their findings in the journal eLife.
To test their new method, the team experimentally engineered yeast, plant, and mammalian cells to contain customizable ligand-binding domains (LBDs), which are receptors for hormones and other types of small molecules. These custom LBDs are tailored so that they only bind and “detect” a specific molecule of interest, such as the human hormone progesterone or the drug digoxin. Once the LBD binds to the target molecule, a secondary “signal” component fused to the LBD can be programmed to emit fluorescence or regulate gene expression. The components of this biosensor — the LBD in combination with the fluorescent or genetic signal — degrade and fade away if the target molecule is not identified.
Strikingly, the team successfully engineered Arabidopsis plants to act as multicellular botanical biosensors, containing a custom LBD to recognize the drug digoxin and a luminescent signal protein to emit light when digoxin is “detected”. These Arabidopsis biosensors gave off fluorescence when the plants were exposed to digoxin, proving that whole organisms can visually light up to signal detection of an arbitrary molecule.
“Like many eukaryotic organisms, plants are full of diverse hormones that make it challenging to sense and respond to a specific hormone of interest,” said Dan Mandell, Ph.D., the study’s co-first author and a Wyss Institute Technology Development Fellow and Postdoctoral Research Fellow at HMS. “But using our strategy, the Arabidopsis plants we engineered exhibited a 50-fold increase in luminescence in the presence of digoxin — very easily visualized — which could inspire exciting future applications involving trees or plants that detect harmful environmental pollutants or toxins and give off a visible indicator.”
“Biosensors that can tell you about their environment are extremely useful for a broad range of applications,” said Church. “You can imagine if they were used in agricultural plants, they can tell you about the condition of the soil, the presence of toxins or pests that are bothering them.”
The team not only demonstrated its novel methodology in plants but also described its efficacy in turning yeast and mammalian cells into precise biosensors, which could one day be leveraged for use in industries that rely on the productivity of yeast or livestock, or for use as medical sensors. Overall, the method is extremely tunable and portable, meaning it can be used in a wide variety of organisms to detect a broad range of small molecules.
An additional capability of the new biosensing methodology is the ability to connect it to gene regulators instead of fluorescent proteins. Such biosensors could precisely regulate gene transcription in order to improve yields of small molecules in organisms used for industrial bioproduction. Yeast, for example, could therefore be engineered to produce a desired molecule from a renewable feedstock, and furthermore programmed to self-identify the most efficient individuals within a population of producers so that only the highest producing yeast would survive. In this way, a population of organisms leveraged for bioproduction of pharmaceuticals or other valuable molecules could quickly self-evolve to become extremely efficient and productive. The team in fact used this strategy to evolve yeast that can produce the hormone progesterone with several-fold higher yield.
The biosensors could have a direct impact on human health as well, given that the team also used their method to tightly regulate the gene editing mechanism CRISPR-Cas9 inside living human cells, a step towards preventing unintended changes to the genome during gene therapy.
“These new reprogramming capabilities developed by the Church team open up an entirely new realm where ordinary organisms can be transformed into extraordinary living cellular devices that can sense specific signals and produce appropriate responses, whether its enhancing production of biofuels or secreting a therapeutic when the cells sense inflammation or infection. It’s another great enabling capability that will undoubtedly advance the entire field of synthetic biology.,” said Wyss Institute Founding Director Donald Ingber, M.D., Ph.D., who is the Judah Folkman Professor of Vascular Biology at HMS and the Vascular Biology Program at Boston Children’s Hospital, as well as Professor of Bioengineering at the Harvard John A. Paulson School of Engineering and Applied Sciences.
The Latest on: Synthetic biology
via Google News
The Latest on: Synthetic biology
- Robot arm ‘tastes’ with engineered bacteriaon July 22, 2019 at 6:52 am
“By combining our work in flexible electronics and robotic skin with synthetic biology, we are closer to future breakthroughs like soft biohybrid robots that can adapt their abilities to sense, feel ... […]
- Newly Discovered Bacterial Natural Product Pathway Offers Potential for Synthetic Biologyon July 22, 2019 at 6:18 am
Shown here are two photos sets of tomato plant leaves, the top set of leaves have no pseudomonas infection and the bottom set of leaves have pseudomonas infection. [Chi P. Ting] Bacteria produce small ... […]
- Synthetic Cannabinoids: The New Age of Medical Marijuana-- CFN Mediaon July 22, 2019 at 5:50 am
The synthetic biology company was formed this year through the merger of BIOCAN Technologies: a team of experienced executives from Calgary and researchers from the University of British Columbia ... […]
- Newly Discovered Biosynthetic Pathway in Bacteria Recipe for Drug Discovery and Productionon July 21, 2019 at 9:38 pm
"We're also excited as to how we might be able to use this for synthetic biology," van der Donk said. "Because the overhead, the amount of resources that have to go in to make a natural product, is ... […]
- Forget synthetic meat, lab grown dairy is hereon July 21, 2019 at 4:47 pm
Five years ago, Perfect Day joined the synthetic biology accelerator IndieBio as it searched for microbes that could be engineered to make functional milk proteins. Today, it has more than 60 ... […]
- Synthetic Biology Market Size |Incredible Possibilities and Growth Analysis and Forecast To 2025on July 20, 2019 at 3:08 am
about Synthetic Biology market report before Buying at:https://www.marketstudyreport.com/enquiry-before-buying/2096022?utm_source=marketwatch.com & utm_medium=PCc ... […]
- Evaluating risks posed by synthetic biologyon July 16, 2019 at 9:04 am
Last week, nearly 70 experts from around the world gathered at EPFL for a workshop on potential threats arising from synthetic biology. Technologies developed in this field aim primarily at treating ... […]
- Could Synthetic Biology Stop Global Warming?on July 16, 2019 at 3:30 am
It's a fact: Latinos are concerned about climate change—actually more than non-Latinos. Producer (and guest host) Antonia Cereijido is no exception and her anxiety led her to the work of Héctor García ... […]
- Global Synthetic Biology Market growing at a CAGR of 28.2% during (2018-2025)-Big Market Researchon July 16, 2019 at 3:03 am
Jul 16, 2019 (Hitech News Daily via COMTEX) -- Synthetic biology is a new interdisciplinary area that involves the application of engineering principles to biology. It aims at the (re-)design and ... […]
- Synthetic Biology Market Volume, Consumption and Prognosticate Industry Growths to 2028on July 15, 2019 at 4:07 am
Jul 15, 2019 (WiredRelease via COMTEX) -- The “Synthetic Biology Market: Worldwide Industrial Study, Size, Share, Growth, Trends, and Forecasts 2019–2028” report offers with all-inclusive, ... […]
via Bing News