New research may revolutionize the slow, cumbersome and expensive process of detecting the antibodies that can help with the diagnosis of infectious and auto-immune diseases such as rheumatoid arthritis and HIV. An international team of researchers have designed and synthetized a nanometer-scale DNA “machine” whose customized modifications enable it to recognize a specific target antibody. Their new approach, which they described this month in Angewandte Chemie, promises to support the development of rapid, low-cost antibody detection at the point-of-care, eliminating the treatment initiation delays and increasing healthcare costs associated with current techniques.
The binding of the antibody to the DNA machine causes a structural change (or switch), which generates a light signal. The sensor does not need to be chemically activated and is rapid – acting within five minutes – enabling the targeted antibodies to be easily detected, even in complex clinical samples such as blood serum.
“One of the advantages of our approach is that it is highly versatile,” said Prof. Francesco Ricci, of the University of Rome, Tor Vergata, senior co-author of the study. “This DNA nanomachine can be in fact custom-modified so that it can detect a huge range of antibodies, this makes our platform adaptable for many different diseases”.
“Our modular platform provides significant advantages over existing methods for the detection of antibodies,” added Prof. Vallée-Bélisle of the University of Montreal, the other senior co-author of the paper. “It is rapid, does not require reagent chemicals, and may prove to be useful in a range of different applications such as point-of-care diagnostics and bioimaging”.
“Another nice feature of our this platform is its low-cost,” said Prof. Kevin Plaxco of the University of California, Santa Barbara. “The materials needed for one assay cost about 15 cents, making our approach very competitive in comparison with other quantitative approaches.”
“We are excited by these preliminary results, but we are looking forward to improve our sensing platform even more” said Simona Ranallo, a PhD student in the group of Prof. Ricci at the University of Rome and first-author of the paper. “For example, we could adapt our platform so that the signal of the nanoswitch may be read using a mobile phone. This will make our approach really available to anyone! We are working on this idea and we would like to start involving diagnostic companies.”
The Latest on: DNA nanomachines
via Google News
The Latest on: DNA nanomachines
- DNA Nanomachines Are Opening Medicine to the World of Physicson October 2, 2019 at 12:00 am
The field has DNA-based devices that “transmit, sense and generate mechanical forces,” the authors said. But eventually, their integration will produce nanomachines that “exert mechanical control over ...
- Scientists reveal DNA packaging mechanism of HSV-1, the virus that causes cold soreson June 13, 2019 at 6:25 am
Credit: University of California, Los Angeles "The DNA packaging process in herpesviruses ... Hong Zhou, director of the Electron Imaging Center for NanoMachines at the California NanoSystems ...
- Ohio State, University of Washington scientists pave way for protein nanomachineson March 18, 2019 at 12:25 pm
Typically, researchers interested in designing biomolecular nanomachines use DNA as the primary component, because complementary DNA strands come together in a highly predictable manner. The research ...
- DNA-based Nanomachines Help Fight Canceron February 9, 2019 at 12:17 pm
New DNA-based nanomachines that can be used for gene therapy of cancer have been proposed by scientists from ITMO in collaboration with their international colleagues. This new invention can ...
- New study determines the fate of DNA derived from genetically modified foodon January 17, 2019 at 11:01 pm
Scientists have published a review of research papers on the fate of the DNA derived from GM food and feed entering the human body and animals. The article published in the journal Food and ...
- Caltech scientists used DNA to play the world’s tiniest game of tic-tac-toeon December 24, 2018 at 10:46 am
“In this study, we invented a new mechanism to program displacement between large DNA tiles, which opens up the possibility to create nanomachines with complex yet reconfigurable parts.
- Scientists program proteins to pair exactlyon December 20, 2018 at 6:48 am
In the past, researchers interested in designing biomolecular nanomachines have often used DNA as a major component. This is because DNA strands come together and form hydrogen bonds to create DNA’s ...
- Molecular hopper lines up for DNA sequencingon October 8, 2018 at 5:00 pm
High-throughput DNA sequencing would likely require the development of longer tracks. More generally, this work represents a significant advancement in the field of nanotechnology and will help to ...
- Controlled nano-assemblyon May 23, 2018 at 8:02 am
His research interests include construction of DNA nanomachines and programmable assembly of bioinorganic nanohybrids.
- Built for speed: DNA nanomachines take a (rapid) step forwardon May 7, 2018 at 5:00 pm
And DNA is the basis for a new breed of tiny robots and nanomachines. Measuring thousands of times smaller than a bacterium, such devices can carry out a multitude of tasks. In new research, Arizona ...
via Bing News