JILA researchers have designed a microscope instrument so stable that it can accurately measure the 3D movement of individual molecules over many hours—hundreds of times longer than the current limit measured in seconds.*
The technology was designed to track the machinery of biological cells, down to the tiniest bits of DNA, a single “base pair” of nucleotides among the 3 billion of these chemical units in human genes. But the instrument could be useful well beyond biology, biochemistry and biophysics, perhaps in manufacturing.
JILA is a partnership of the National Institute of Standards and Technology (NIST) and the University of Colorado Boulder.
“This technology can actively stabilize two items relative to each other with a precision well below one nanometer at room temperature,” JILA/NIST physicist Tom Perkins says. “This level of 3D stability may start to interest the nanomanufacturing world, when they look at making and characterizing things on the single-nanometer scale.”
The work builds on JILA’s world-leading expertise in measuring positions of microscopic objects. The latest tweaks extend stability for a much longer time period, many hours at a time. With the longer observation times, researchers can see more successive steps of molecular motors, for instance. These biochemical processes are responsible for a broad range of movement in living organisms, including moving molecules around the interior of a cell or copying DNA into another form of genetic material, RNA. The new JILA instrument also can aid in measuring individual proteins as they fold into specific positions, a process required for them to work properly.
Until now, researchers had difficulty detecting more than a few individual, one-base-pair steps in succession before instrumental “drift” would blur the signal. Observing such sets of repetitive steps is very rare. The instrument must be stable to within about one-tenth of a nanometer (1 angstrom to biologists, equivalent to the diameter of a hydrogen atom).
Typically, a microscope can only occasionally achieve this level of stability. But when augmented by the new JILA measurement platform, it can reliably achieve tenth of a nanometer stability for up to 100 seconds at a time. And it can do this over and over again for extended periods—the JILA team operated the system for up to 28 hours straight.
In addition to its high precision and stability, the instrument can detect motion over a wide range of time scales, critical for calibrating instruments and measuring short-lived states in protein folding. The JILA method can be applied to optical trapping techniques, atomic force microscopes and super-resolution imaging.
The Latest on: Ultra-stable Microscopy
via Google News
The Latest on: Mltra-stable Microscopy
- Ground-breaking Modular Research Microscope, Showcasing in PI Booth at Neuroscience 2019on October 18, 2019 at 12:49 pm
PI provides a large variety of fast XYZ stages and microscope lens positioners for 3D imaging (Z-stack acquisition), deconvolution, and fast auto-focusing applications. A line of ultra-stable ...
- Nikon Releases High-Speed Super Resolution Microscopeon August 6, 2018 at 7:00 am
Ultra-stable platform for live-cell super-resolution imaging The N-SIM S takes advantage of the ultra-stable ECLIPSE Ti2-E Inverted Research Microscope platform designed for super-resolution imaging.
- Short-Arc Mercury Lamp for Microscopy Systemson July 29, 2018 at 5:00 pm
Ushio’s USH-103D short-arc mercury lamps are designed for use in all 100W fluorescent microscope systems ... high luminance, and ultra-stable arc, spectral outputs in the ultraviolet and visible light ...
- Chapter 10.9: Confocal Microscopyon February 21, 2018 at 2:02 pm
With numerous algorithm illustrations as well as tutorial chapters, this guide contains a comprehensive presentation of all essential mathematics, techniques, and algorithms for every type of image ...
- K: Kerr Microscopy Kondo Systems and Heavy Fermions: Transport Phenomenaon February 21, 2018 at 1:18 am
Kerr microscopy allows the imaging of magnetic domains and magnetization processes in an optical polarization microscope. The method is based on the magnetooptical Kerr effect, i.e., the rotation of ...
- 2016 Neuroscience Conference Debuts PI's Ultra-Stable Microscope XY Stage Packageson November 2, 2016 at 5:00 pm
Physik Instrumente will introduce two new compact XY microscope stages based on ultrasonic piezo direct-drive motors. Model U-761 provides 25x25mm travel and is equipped with 10nm resolution linear ...
- Ultra-stable JILA microscopy technique tracks tiny objects for hourson July 1, 2015 at 6:39 am
JILA researchers have designed a microscope instrument so stable that it can accurately measure the 3D movement of individual molecules over many hours—hundreds of times longer than the current limit ...
- JEOL and UC Irvine Partner to Develop Premier Electron Microscopy and Materials Research Centeron January 13, 2015 at 8:45 am
and an ultra-stable cold-cathode field emission electron gun. The center will also house the high throughput, nano-analysis JEM-2800 TEM/STEM, a versatile microscope favored for its ease of use while ...
- World's most powerful microscope ready for researchon June 17, 2013 at 5:00 pm
(Phys.org) —The world's most powerful microscope, which resides in a specially constructed ... A team from Hitachi, which constructed the ultra high-resolution, ultra-stable instrument, spent one year ...
via Bing News