A new gene sequencing technology known as ‘Capture Sequencing’ (CaptureSeq) allows us to explore the human genome at a much higher resolution than ever before, with revolutionary implications for research and cancer diagnosis.
A new gene sequencing technology allows us to explore the human genome at a much higher resolution than ever before, with revolutionary implications for research and cancer diagnosis.
Outperforming existing technologies, ‘Capture Sequencing’ (CaptureSeq) can accurately measure the activity of many specific genes in a sample – even when they are expressed at minute levels. This will have immediate practical applications, including the diagnosis of many blood cancers.
Publishing today in the leading journal Nature Methods, an Australian team demonstrated the strengths of CaptureSeq.
Dr Tim Mercer, Dr Michael Clark, Professor John Mattick and Associate Professor Marcel Dinger from Sydney’s Garvan Institute of Medical Research, who developed CaptureSeq, compared it with techniques currently in use for gene expression analysis, and found it to be much more sensitive to genes expressed at very low levels.
Until recently, it was thought that humans have around 20,000 genes – that is, the sections of DNA that are ‘transcribed’ into RNA molecules, then ‘translated’ into the proteins that perform tasks in cells.
Protein-coding genes occupy only 2% of the genome, and their number and function remains relatively constant across the animal kingdom, from worms to humans.
We now understand that the rest of the genome is made up of genes that do not code for proteins, and that these non-coding genes create the complexity that distinguishes humans from worms.
Non-protein-coding genes perform an important regulatory function in various aspects of human development and brain function. Most are expressed only in a few cells rather than whole tissues, or they are expressed at very low levels, making them difficult to study.
The new study shows that CaptureSeq will help us uncover these rare genes by allowing us to explore specific stretches of the human genome at much higher resolution than current RNA sequencing approaches.
CaptureSeq will also enable rapid detection of diseases where diagnosis is guided by gene expression, and the genes involved are known.
The Latest on: Capture Sequencing
via Google News
The Latest on: Capture Sequencing
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- Virome capture sequencing does not identify active viral infection in unicentric and idiopathic multicentric Castleman disease.on August 2, 2019 at 5:00 pm
Nabel CS; Sameroff S; Shilling D; Alapat D; Ruth JR; Kawano M; Sato Y; Stone K; Spetalen S; Valdivieso F; Feldman MD; Chadburn A; Fosså A; van Rhee F; Lipkin WI; Fajgenbaum DC Castleman disease (CD) ...
- The Rise of Long-Read Transcriptome Sequencingon July 11, 2019 at 8:27 am
While short-read sequencing power stems from large read output and high ... The rise of long-read technologies now opens the possibility to fully capture a full-length transcript within a single read ...
- BARDA Awards Contract to DNAe to Develop Semiconductor DNA Sequencing Platformon May 29, 2019 at 5:00 pm
The contract for the project, entitled PISCES (Pathogen Identification from Specimen, via Capture Extraction and Sequencing), will enable DNAe to complete the development and validation of its ...
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