Researchers have shown that existing optical fibre technology could be used to produce microscopic 3D images of tissue inside the body, paving the way towards 3D optical biopsies.
Unlike normal biopsies where tissue is harvested and sent off to a lab for analysis, optical biopsies enable clinicians to examine living tissue within the body in real-time.
This minimally-invasive approach uses ultra-thin microendoscopes to peer inside the body for diagnosis or during surgery, but normally produces only two-dimensional images.
Research led by RMIT University has now revealed the 3D potential of the existing microendoscope technology.
Published in Science Advances, the development is a crucial first step towards 3D optical biopsies, to improve diagnosis and precision surgery.
Lead author Dr Antony Orth said the new technique uses a light field imaging approach to produce microscopic images in stereo vision, similar to the 3D movies that you watch wearing 3D glasses.
“Stereo vision is the natural format for human vision, where we look at an object from two different viewpoints and process these in our brains to perceive depth,” said Orth, a Research Fellow in the RMIT node of the ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP).
“We’ve shown it’s possible to do something similar with the thousands of tiny optical fibres in a microendoscope.
“It turns out these optical fibres naturally capture images from multiple perspectives, giving us depth perception at the microscale.
“Our approach can process all those microscopic images and combine the viewpoints to deliver a depth-rendered visualization of the tissue being examined – an image in three dimensions.”
How it works
The research revealed that optical fibre bundles transmit 3D information in the form of a light field.
The challenge for the researchers was then to harness the recorded information, unscramble it and produce an image that makes sense.
Their new technique not only overcomes those challenges, it works even when the optical fibre bends and flexes – essential for clinical use in the human body.
The approach draws on principles of light field imaging, where traditionally, multiple cameras look at the same scene from slightly different perspectives.
Light field imaging systems measure the angle of the rays hitting each camera, recording information about the angular distribution of light to create a “multi-viewpoint image”.
But how do you record this angular information through an optical fibre?
“The key observation we made is that the angular distribution of light is subtly hidden in the details of how these optical fibre bundles transmit light,” Orth said.
“The fibres essentially ‘remember’ how light was initially sent in – the pattern of light at the other side depends on the angle at which light entered the fibre.”
With this in mind, RMIT researchers and colleagues developed a mathematical framework to relate the output patterns to the light ray angle.
“By measuring the angle of the rays coming into the system, we can figure out the 3D structure of a microscopic fluorescent sample using just the information in a single image,” Professor Brant Gibson, Chief Investigator and Deputy Director of the CNBP, said.
“So that optical fibre bundle acts like a miniaturised version of a light field camera.
“The exciting thing is that our approach is fully compatible with the optical fibre bundles that are already in clinical use, so it’s possible that 3D optical biopsies could be a reality sooner rather than later.”
In addition to medical applications, the ultra-slim light field imaging device could potentially be used for in vivo 3D fluorescence microscopy in biological research.
The Latest on: 3D optical biopsies
via Google News
The Latest on: 3D optical biopsies
- Optical Coherence Tomography in Cornea and Refractive Surgeryon October 3, 2020 at 5:00 pm
SS-1000 Casia Tomey Spectral-domain OCT 10 µm Prepares anterior and posterior corneal elevation maps and 3D images of the ... cross-sectional optical biopsy of the ocular tissues.
- Quantum Cascade Laser Market Size Worth USD 451.3 Million by 2027 | CAGR of 3.8%: Emergen Researchon October 1, 2020 at 11:14 am
This is particularly helpful in liver biopsy. QCL is also used to develop ... UV - C), By Applications (Optical Sensors and Instrumentation, Counterfeit Detection, UV Sterilization, Medical ...
- The Light Stuff: Optical Imaging in Medical Diagnosison September 29, 2020 at 5:00 pm
These answers, if they come, will be derived through optical biopsies, a fringe technique that uses visible or near-infrared light to examine in vivo tissue. The technology promises more accurate, ...
- Recent Advances in Optical Coherence Tomography for the Diagnoses of Lung Disorderson September 28, 2020 at 5:00 pm
Electromagnetic navigation bronchoscopy (ENB) is a bronchoscopic technique that uses an electromagnetic field and a 3D CT reconstruction ... and a needle or biopsy forceps is inserted through ...
- Biopsy Devices Pipeline Insight Market Research Reporton September 20, 2020 at 10:06 am
Significant increase in incidence of cancer, such as breast, prostate, and lung, and rapid technological advancements such as 3D optical biopsies, MRI-targeted biopsies, and Ultrasound-guided ...
- Contactless 3D body scanner to shed light on diagnosison September 8, 2020 at 3:46 am
The world’s first contactless 3D body scanner is being developed in the UK to aid ... Researchers from these institutions will use advanced optical physics, detector technology and Artificial ...
- Corneal Diseases of Dogs and Catson August 14, 2020 at 11:26 am
The transparency of the cornea is based on the lack of blood vessels and cells, the lack of pigment, the control of corneal water content, and the smooth optical surface afforded ... but ultimately ...
- Pentascope/Pentastar Colposcope from Wallachon August 3, 2017 at 6:03 pm
Wallach’s highly versatile PentaScope/PentaStar series offers an optical ... the 3D image needed to detect subtle tissue abnormalities and to perform colposcopically directed biopsies and ...
- Researchers Present Wide Variety of New Quantum Toolson May 9, 2016 at 3:23 pm
Malvin Teich of Boston University presented a new twist in a 3D diagnostic imaging technique known as optical coherence tomography (OCT), widely used in opthalmology and in creating cross-section ...
via Bing News