UC Riverside study uses gene-editing tool to disrupt serotonin receptor linked to egg production in Aedes aegypti mosquitoes
Mosquitoes are the world’s deadliest animals, killing thousands of people and causing millions of illnesses each year. To be able to reproduce and become effective disease carriers, mosquitoes must first attain optimal body size and nutritional status.
A pair of researchers at the University of California, Riverside, have succeeded in using CRISPR-Cas9, a powerful tool for altering DNA sequences and modifying gene function, to decrease mosquito body size, moving the research one step closer to eliminating mosquitoes that carry dengue fever and Zika virus.
The researchers succeeded in postponing mosquito development, shortening the animal’s lifespan, retarding egg development, and diminishing fat accumulation.
Alexander Raikhel, a distinguished professor of entomology, and Lin Ling, a postdoctoral scholar working with Raikhel, used CRISPR-Cas9 to disrupt the serotonin receptor Aa5HT2B in Aedes aegypti mosquitoes, the vectors of dengue fever, yellow fever, and Zika virus.
“Aa5HT2B controls insulin-like peptides,” Raikhel said. “We were able to uncover the different roles that these peptides play in controlling body size and metabolism, and disrupt the gene associated with this receptor.”
The team accomplished this, Raikhel said, by uncovering a key molecular pathway determining mosquito body size and metabolism.
“Mosquitoes of small size with diminished fat resources mature later and live shorter lives than nonmodified mosquitoes,” he said. “Thus, these genetically engineered mosquitoes have low reproductive capacity and ability to transmit disease pathogens. These features of CRISR-Cas9 mutant mosquitoes can be exploited for developing novel mosquito control approaches. Many challenges remain on the road, however, toward achieving this goal.”
Study results appear in the Proceedings of the National Academy of Sciences.
Raikhel, the UC Presidential Chair and the Mir Mulla Endowed Chair in the Department of Entomology and a member of the National Academy of Sciences, explained that disease-transmitting female mosquitoes require a vertebrate blood meal to produce their eggs because egg development occurs only after a diet change from carbohydrate-rich nectar to protein-rich vertebrate blood.
Blood feeding, Raikhel added, boosts serotonin concentration and increases the level of the serotonin receptor Aa5HT2B in the “fat-body,” the insect analog of vertebrate liver and adipose tissue. A target for hormones, the fat-body is the main nutrient sensor in insects. It links nutritional state, metabolism, and growth.
“Our study provides for the first time a link — the serotonin receptor Aa5HT2B — between blood feeding and the serotonin signaling that is specific to the fat-body,” he said. “Aa5HT2B mediates serotonin action. Until now, the mechanisms of serotonin action specific to the fat-body were poorly understood. Understanding regulatory mechanisms that underlie determination of body size and metabolism is important for developing novel approaches to control mosquito populations and the diseases they carry.”
One important question for further research is how CRISPR-Cas9 gene modification could be introduced into the wild mosquito population.
“This question is a topic of intense research in other laboratories,” Raikhel said. “At UCR, we are continuing our efforts in identifying other key processes important for mosquito development that could be exploited for mosquito control.”
The Latest on: Mosquito control
via Google News
The Latest on: Mosquito control
- Livingston council members make plans, face questions about May 4 mosquito control proposition on February 20, 2019 at 9:22 pm
WATSON — A proposed mosquito abatement program in western Livingston Parish would provide education, surveillance, treatment and personal service, but is already facing opposition with two months to g... […]
- Tanzanian research institute develops repellent to control mosquitoes on February 20, 2019 at 3:53 pm
Tanzania’s Ifakara Health Institute (IHI) has developed a repellent to control mosquitoes that spread dengue, Chikunganya and Zika diseases, a senior research scientist revealed on Monday. Nico Govell... […]
- Flies, Ants, Mosquitoes Among Top-Searched Pests in the U.S. on February 20, 2019 at 10:34 am
The study analyzed Google's search interest data of 30 common household pests and rodents including flies, ants, bed bugs, mosquitoes, fleas, mice, and cockroaches. The study specifically focused on p... […]
- Making Malaria Mosquitoes Extinct Using Engineered Gene-Drives on February 20, 2019 at 9:35 am
The technology would also work to control mosquito species that infect people with maladies like Zika virus and dengue fever. FOE, along with numerous other anti-technology activist groups ... […]
- Scientists Are Putting Mosquitoes on Human Diet Drugs on February 19, 2019 at 10:37 am
Humans and mosquitoes hardly have anything in common—and certainly not our gustatory tastes or dietary habits. But it turns out that we share some common pathways to satiety—in particular the neural r... […]
- Orlando School Hosts Mosquito Invasion on February 19, 2019 at 7:40 am
Mosquito control experts from across the country will be visiting Montessori World School to offer students an unforgettable interactive science experience. More than a dozen mosquito professionals fr... […]
- American Mosquito Control Association Orlando Meeting to Highlight Emerging Mosquito Diseases on February 19, 2019 at 6:39 am
ORLANDO, Fla., Feb. 19, 2019 /PRNewswire-PRWeb/ -- The American Mosquito Control Association (AMCA) will hold their 85th Annual Meeting at the Caribe Royale Resort Feb. 25-Mar. 1, 2019. The meeting wi... […]
- The key to control mosquito menace is to figure out the root cause, say experts on February 18, 2019 at 4:26 pm
KOCHI: As Kochi witnessed a sudden surge in mosquito menace, it's time to delve deep into the cause. While spending tax payer's money on prevention fails to yield results, experts have mooted sustaina... […]
- Judge to settle pesticide dispute in mosquito-prone area on February 18, 2019 at 9:23 am
GOSHEN, Vt. (AP) — A Vermont judge is going to settle a dispute between a mosquito-control district and an environmental group appealing the pesticide permit awarded to the group by the state. The Rut... […]
via Bing News