Purdue University researchers have identified a new compound that in preliminary testing has shown itself to be as effective as antibiotics approved by the Food and Drug Administration to treat life-threatening infections while also appearing to be less susceptible to bacterial resistance.
The compound, called F6, has been potent against antibiotic-resistant pathogens such as methicillin-resistant Staphylococcus aureus (MRSA), which is often found in hospitals and other health care settings, and vancomycin-resistant Staphylococcus aureus (VRSA), with vancomycin long considered a drug of last resort. The compound was tested against clinical isolates.
“This is very exciting,” said Herman Sintim, drug discovery professor in Purdue’s Department of Chemistry. “We are not the first to report of a new molecular entity that killed these drug-resistant pathogens. But what is unique about the compound that we found is that when we tried to generate resistance in the lab, we couldn’t.”
Antibiotic resistance is a growing public health crisis. The World Health Organization has deemed antibiotic resistance one of the three greatest threats to human health because bacteria are becoming increasingly resistant and too few treatments are being developed. The Centers for Disease Control and Prevention reports that at least 2 million people a year in the United States become infected with bacteria resistant to antibiotics and at least 23,000 people die a year as a result. Studies have estimated that drug-resistant infections could be responsible for 10 million deaths a year worldwide by 2050.
Pharmaceutical companies have been reluctant to invest in antibiotics because it typically costs millions of dollars to develop a drug and the probability of bacterial resistance is high.
Purdue researchers identified F6 by screening a chemical library for compounds with antibacterial activity. They tried to force bacteria resistance on F6, performing experiments to evaluate the ability of MRSA USA400 to develop resistance to F6 in vitro.
“The idea is that if you keep adding increasing concentrations to bacteria and then you keep regrowing the bacteria, after so many cycles you are going to develop resistance,” Sintim said. “Scientists do this to figure out whether whatever they have created develops resistance quickly.”
The minimal inhibitory concentration, or MIC, remained unchanged for F6 over nine passages and doubled on the10th passage. It then remained unchanged up to the 14th passage during a two-week period. By comparison, the MIC of the antibiotic ciprofloxacin tripled after the eighth passage and continued to rapidly increase to more than 2,000-fold by the 14th passage.
“We are not saying there will never be resistance to the F6 molecule or analogs thereof. What we are saying is that here is a new molecule that works and when we try to force resistance we couldn’t generate resistance,” Sintim said.
F6, which is nontoxic to humans and other mammals, works against bacteria in a group known as Gram-positive, but not against those that are Gram-negative. F6 was effective against MRSA, VRSA, Enterococcus faecalis, which lives in the human gut, vancomycin-resistant Enterococcus (VRE) and Listeria monocytogenes, often associated with unpasteurized dairy products.
Testing on mice also indicated F6 was as effective as fusidic acid in treating a wound infected with MRSA, further confirming its potent antibacterial effect.
The Purdue researchers will now begin to make derivatives of F6 to see if they might be even more effective. They are working with the Purdue Office of Technology Commercialization to look for partners to help further develop this exciting new molecular entity.
The Latest on: Antibiotic-resistant pathogens
via Google News
The Latest on: Antibiotic-resistant pathogens
- Study identifies how enterococci bacteria cause antibiotic-resistant bloodstream infections on April 17, 2019 at 7:41 pm
A new study led by a research team from Massachusetts Eye and Ear and Harvard Medical School describes how bacteria adapted to the modern hospital environment and repeatedly cause antibiotic-resistant ... […]
- How Enterococcus faecalis bacteria causes antibiotic resistant infection on April 17, 2019 at 8:58 am
A new study describes how bacteria adapted to the modern hospital environment and repeatedly cause antibiotic-resistant bloodstream infections. This study examined one of the first sustained hospital ... […]
- Wastewater treatment plants harbor antibiotic-resistant genes on April 17, 2019 at 3:09 am
“Similarly, people living in the south of Europe tend to carry a much higher number of antibiotic-resistant bacteria than those living in northern Europe and that is seen in wastewaters.” Specifically ... […]
- German chicken meat contaminated with antibiotic-resistant superbugs on April 16, 2019 at 11:06 pm
More than half of chicken meat sold at low-cost supermarkets in Germany is contaminated with antibiotic-resistant bacteria, according to a probe of meat samples. The environmental and consumer ... […]
- Hospital patients' hands are covered in antibiotic resistant superbugs, study suggests on April 16, 2019 at 4:25 am
and the team found that 29 percent of them had populations of resistant bacteria. Six percent of patients picked up new antibiotic resistant germs on their hands over the courses of their stays at the ... […]
- Genetic ‘weapon’ picks off pathogens — but spares beneficial microbes on April 15, 2019 at 1:46 pm
These switches allow toxin to be produced only after the plasmids are nestled inside antibiotic-resistant Vibrio cholerae, the bacterium that causes cholera. The researchers then loaded these plasmid ... […]
- Novel Weapon to Target Antibiotic Resistant Bacteria on April 15, 2019 at 8:29 am
Antibiotic resistance could be addressed through a new tool developed by the scientists from the Institut Pasteur, the CNRS and the Universidad Politécnica de Madrid by programming a bacterial genetic ... […]
- A new bacteria-killing weapon in the fight against antibiotic resistance on April 15, 2019 at 8:05 am
the CNRS and the Universidad Politécnica de Madrid successfully programmed a bacterial genetic structure to make it capable of specifically killing multiple antibiotic-resistant bacteria without ... […]
- Special antimicrobial soap used to fight antibiotic-resistant superbugs in hospitals and nursing homes on April 14, 2019 at 12:36 pm
At least 2 million people in the U.S. become infected with an antibiotic-resistant bacterium each ... Some of the most common bacteria in health care facilities are methicillin-resistant ... […]
via Bing News