Purdue University researchers have identified a new compound that in preliminary testing has shown itself to be as effective as antibiotics approved by the Food and Drug Administration to treat life-threatening infections while also appearing to be less susceptible to bacterial resistance.
The compound, called F6, has been potent against antibiotic-resistant pathogens such as methicillin-resistant Staphylococcus aureus (MRSA), which is often found in hospitals and other health care settings, and vancomycin-resistant Staphylococcus aureus (VRSA), with vancomycin long considered a drug of last resort. The compound was tested against clinical isolates.
“This is very exciting,” said Herman Sintim, drug discovery professor in Purdue’s Department of Chemistry. “We are not the first to report of a new molecular entity that killed these drug-resistant pathogens. But what is unique about the compound that we found is that when we tried to generate resistance in the lab, we couldn’t.”
Antibiotic resistance is a growing public health crisis. The World Health Organization has deemed antibiotic resistance one of the three greatest threats to human health because bacteria are becoming increasingly resistant and too few treatments are being developed. The Centers for Disease Control and Prevention reports that at least 2 million people a year in the United States become infected with bacteria resistant to antibiotics and at least 23,000 people die a year as a result. Studies have estimated that drug-resistant infections could be responsible for 10 million deaths a year worldwide by 2050.
Pharmaceutical companies have been reluctant to invest in antibiotics because it typically costs millions of dollars to develop a drug and the probability of bacterial resistance is high.
Purdue researchers identified F6 by screening a chemical library for compounds with antibacterial activity. They tried to force bacteria resistance on F6, performing experiments to evaluate the ability of MRSA USA400 to develop resistance to F6 in vitro.
“The idea is that if you keep adding increasing concentrations to bacteria and then you keep regrowing the bacteria, after so many cycles you are going to develop resistance,” Sintim said. “Scientists do this to figure out whether whatever they have created develops resistance quickly.”
The minimal inhibitory concentration, or MIC, remained unchanged for F6 over nine passages and doubled on the10th passage. It then remained unchanged up to the 14th passage during a two-week period. By comparison, the MIC of the antibiotic ciprofloxacin tripled after the eighth passage and continued to rapidly increase to more than 2,000-fold by the 14th passage.
“We are not saying there will never be resistance to the F6 molecule or analogs thereof. What we are saying is that here is a new molecule that works and when we try to force resistance we couldn’t generate resistance,” Sintim said.
F6, which is nontoxic to humans and other mammals, works against bacteria in a group known as Gram-positive, but not against those that are Gram-negative. F6 was effective against MRSA, VRSA, Enterococcus faecalis, which lives in the human gut, vancomycin-resistant Enterococcus (VRE) and Listeria monocytogenes, often associated with unpasteurized dairy products.
Testing on mice also indicated F6 was as effective as fusidic acid in treating a wound infected with MRSA, further confirming its potent antibacterial effect.
The Purdue researchers will now begin to make derivatives of F6 to see if they might be even more effective. They are working with the Purdue Office of Technology Commercialization to look for partners to help further develop this exciting new molecular entity.
The Latest on: Antibiotic-resistant pathogens
via Google News
The Latest on: Antibiotic-resistant pathogens
- Novel approach leads to potential sepsis prevention in burn patientson September 6, 2019 at 5:15 pm
Pseudomonas aeruginosa, an opportunistic pathogen, is among the different pathogens that cause sepsis in burn patients. This gram-negative, antibiotic-resistant bacterium produces several destructive ...
- 80% cut in antibiotics entering Thames is needed to avoid surge in superbugson September 6, 2019 at 8:21 am
It found that across three-quarters of the Thames catchment, the antibiotics present, due to effluent discharge, were likely to be at levels high enough for antibiotic-resistant bacteria to develop.
- Thames typical of superbug-breeding rivers, says new studyon September 5, 2019 at 2:41 pm
The River Thames is rapidly becoming a breeding pool for antibiotic-resistant bacteria, according to a new study, which underlines the urgent need to cut down the amount of antibiotics currently being ...
- 4 Facts Parents Should Know About Antibiotic Resistanceon September 5, 2019 at 10:00 am
Research from Evivo baby probiotic confirms newborns just 7 days old are born with high levels of antibiotic-resistant bacteria and release millions of resistant bacteria in each dirty diaper ...
- ContraFect to Present at the 2019 Janney Healthcare Conferenceon September 4, 2019 at 5:30 pm
Amurin peptides are new class of DLAs which exhibit broad-spectrum activity against a wide range of antibiotic-resistant Gram-negative pathogens, including Pseudomonas aeruginosa (P. aeruginosa ...
- In Urgent Care, Don't Forget the Pharmaciston September 4, 2019 at 1:02 pm
With an increasing rate of inappropriate antibiotic prescribing, how is it possible to achieve the goal of the U.S. National Action Plan for Combating Antibiotic-Resistant Bacteria, which is to reduce ...
- New study may help the battle against a pathogenic bacteriumon September 4, 2019 at 6:34 am
“It might be that there are less strong selective pressures for bacteria to overcome a drug that just makes them disoriented,” Guillemin said. “By 2050 there’s going to be pandemics of antibiotic ...
- Bacteria can become antibiotic-resistant by closing pores on their surface to stop drugs getting inon September 1, 2019 at 10:00 pm
Scientists have discovered how bacteria become resistant to antibiotics and say they may be able to work out how to get past their defences. Antibiotic-resistant bacteria, known as superbugs, are a ...
- Molecular mechanism by which human stomach pathogen is attracted to bleach revealedon August 30, 2019 at 12:47 am
"It might be that there are less strong selective pressures for bacteria to overcome a drug that just makes them disoriented," Guillemin said. "By 2050 there's going to be pandemics of ...
via Bing News