Oct 202017

Kawaoka gives a slide presentation to a group of media representatives touring the Influenza Research Institute (IRI) at UW–Madison in February 2017. The high-security research facility was closed down for annual decontamination, cleaning and maintenance. PHOTO: JEFF MILLER

In 2013, an influenza virus that had never before been detected began circulating among poultry in China. It caused several waves of human infection and in late 2016, the number of people to become sick from the H7N9 virus suddenly started to rise. As of late July 2017, nearly 1,600 people had tested positive for avian H7N9. Nearly 40 percent of those infected had died.

In early 2017, Yoshihiro Kawaoka, professor of pathobiological sciences at the University of Wisconsin–Madison School of Veterinary Medicine, received a sample of H7N9 virus isolated from a patient in China who had died of the flu. He and his research team subsequently began work to characterize and understand it. The first of those results are published today (Oct. 19, 2017) in Cell Host & Microbe.

For the first time, Kawaoka says, his team has identified an influenza virus strain that is both transmissible between ferrets (the best animal model proxy for human influenza infections) and lethal, both in the animal originally infected and in otherwise healthy ferrets in close contact with these infected animals.

“This is the first case of a highly pathogenic avian virus that transmits between ferrets and kills them,” Kawaoka says. “That’s not good for public health.”

Everyone in the influenza field knew it was only a matter of time before the virus became pathogenic in chickens, which is to say that it became capable of causing disease, but Kawaoka says it took several years. It was initially hard to detect because, unlike some other influenza viruses such as H5N2 — which is highly lethal in chickens and caused significant outbreaks on poultry farms across the U.S. and elsewhere in 2015 — H7N9 was not killing the chickens it infected.

Instead, it remained silent, passing unknown from chicken to chicken and, occasionally, infecting humans that came into contact with the birds.

Influenza viruses are well known for their propensity to adapt. With each new infection of a host, small changes take place within the genomes of influenza viruses. Sometimes these mutations occur in key regions and lead to significant alterations to the original virus, rendering it capable of infecting new hosts, making hosts sick, causing greater illness, and becoming resistant to the drugs typically used to treat them.

Kawaoka and his team observed this within the sample isolated from the deceased patient, who while alive had been treated with the common flu drug Tamiflu. Using a technique to read the genetic identity of the virus population that had infected the patient, Kawaoka’s team learned the virus had started to mutate: The sample contained a population of H7N9 virus that was sensitive to Tamiflu and a population that was resistant.

So the team created two viruses virtually identical to those isolated from the patient, one sensitive to Tamiflu and the other bearing the mutation that conferred resistance to the drug. Comparing this to a low-pathogenic version of the H7N9 virus that Kawaoka and others had previously studied, the research team assessed how well each virus grows in human respiratory cells, where most influenza viruses take up residence in the body. They found that each grew efficiently, though the resistant strain was less effective than the other two.

The team also found that each virus infects and causes illness, to varying degrees, in several animal models for influenza — mice, ferrets and macaques.

To test whether the virus was transmissible between mammals, the researchers set up experiments in which ferrets were housed alone in individual cages separated by a barrier that allowed respiratory droplets to pass from one cage to the next. In each pair, one ferret was deliberately infected with the virus while the other was placed into the cage healthy.

Each of the three virus types were transmitted from infected ferrets to the previously uninfected animals. Two of three ferrets infected with the nonresistant strain of H7N9 — the strain currently circulating in China — died, as did the animals to which they passed the virus.

“Without additional mutations, the virus transmitted and killed ferrets,” says Kawaoka, noting that further alterations to the virus may not be necessary to make it a potential public health threat, though human-to-human transmission has thus far remained limited.

The team also confirmed the drug-resistant H7N9 did not respond to oseltamivir, the active agent in Tamiflu. It did respond to another drug called a protease inhibitor, but Kawaoka says it is a drug currently approved only in Japan and only for use in pandemic situations.

“I don’t want to cause alarm,” Kawaoka says, but “it’s only a matter of time before the resistant virus acquires a mutation that allows it to grow well, (rendering it) more likely to be lethal at the same time it is resistant.”

However, Kawaoka and his team are currently unable to better understand what mutations may enable this transition, at least in the United States, where a moratorium on work that might cause a pathogen to take on a new function not currently known in nature has been in place for several years.

“We can’t do the experiments to find out why,” Kawaoka says. “We really need to understand why H7N9 is lethal and transmissible, and what is different in this one resistant H7N9. If we knew that, because there are multiple viruses circulating, we could narrow down efforts to those that are lethal and transmissible.”

He recently published a commentary in the Proceedings of the National Academy of Sciences, co-authored with two colleagues who are also experts in influenza, in which they explain the challenges this moratorium creates for understanding the potential of viruses like H7N9 to become pandemic.

“Results from (gain-of-function) studies would almost certainly help in understanding the pandemic potential of influenza viruses and produce public health benefits, such as the prioritization and development of pre-pandemic vaccines and antiviral drugs,” the authors write. Fundamental (gain-of-function) research on transmissibility, host-range restriction, drug resistance, immunogenicity, pathogenicity, and replicative ability would also benefit global public health.”

The H7N9 virus is likely to continue to mutate as it infects humans, resulting in adaptations that enhance the viruses’ pathogenicity or ability to pass from person to person, Kawaoka adds. In other words, nature is already performing its own gain-of-function experiments, with potentially serious consequences.

It has, however, become a bit easier recently to detect when poultry are infected with H7N9, thereby allowing people to limit their exposure. That’s because the virus has begun to kill birds in China, too. But unlike in the U.S., where farmers cull their flocks to limit the spread of infectious disease, China relies on vaccines. This worries Kawaoka, given how well the virus has been shown to grow.

For now, he says: “We should improve our surveillance.”

Learn more: H7N9 influenza is both lethal and transmissible in animal model for flu


The Latest on: H7N9 influenza
  • According to new reports, bird flu is back. Here's what you need to know.
    on November 21, 2017 at 12:52 pm

    Now, it seems, it’s back. Global health officials are warning that there is a concerning bird flu circulating throughout Asia. Known as the H7N9 virus, this form of bird flu is in its “fifth wave,” per the Centers for Disease Control and Prevention. […]

  • CDC Centers for Disease Control and Prevention : Research on Zoonotic (Animal Origin) Influenza (Flu) Viruses of Public Health Concern
    on November 21, 2017 at 6:16 am

    For example, Asian lineage highly pathogenic avian influenza (HPAI)* A(H5N1) or Asian lineage A(H7N9) viruses are zoonotic flu viruses of public health concern because they have infected people and caused serious illness. Zoonotic origin flu viruses that ... […]

  • Asia’s Bird Flu Putting Global Health Officials On High Alert
    on November 21, 2017 at 3:32 am

    global health agencies are steadily ratcheting up concern about bird flu in Asia. Bird viruses that can infect humans — particularly those of the H7N9 strain — continue to spread to new cities there. Since October 2016, China has seen a “fifth wave ... […]

  • Ready for a Global Pandemic?
    on November 21, 2017 at 12:00 am

    Consider the current H7N9 bird flu epidemic in China. It has infected more than 1,600 people since 2013, with a fatality rate of 40 percent. Although humans have contracted it mostly through contact with infected poultry, it is possible that limited person ... […]

  • Bird Flu Is Spreading in Asia, Experts (Quietly) Warn
    on November 19, 2017 at 9:32 pm

    While trying to avoid alarmism, global health agencies are steadily ratcheting up concern about bird flu in Asia. Bird viruses that can infect humans — particularly those of the H7N9 strain — continue to spread to new cities there. Since October 2016 ... […]

  • This Canadian company is on the forefront of global efforts to stop the next pandemic
    on November 17, 2017 at 9:45 am

    Currently, the bird flu virus called H7N9 is being carefully watched, as the United States Centers for Disease Control and Prevention (CDC) calls H7N9 a strain with the greatest potential to cause a pandemic, one that could result in deaths in the tens of ... […]

  • What You Need to Know About Bird Flu
    on November 11, 2017 at 3:21 am

    Some strains of bird flu (namely the H7N9 variety) appear to have become increasingly virulent to humans—the outbreak earlier this year in China is one example. But the Centers for Disease Control says that overall, human cases are still rare and sporadic. […]

  • New H7N9 bird flu strain in China has pandemic potential: study
    on October 19, 2017 at 12:54 pm

    CHICAGO (Reuters) - Lab experiments on a new strain of the H7N9 bird flu circulating in China suggest the virus can transmit easily among animals and can cause lethal disease, raising alarm that the virus has the potential to trigger a global human ... […]

  • H7N9 bird flu has the makings of a pandemic virus, scientists warn
    on March 11, 2015 at 3:28 pm

    Tests of H7N9 influenza strains in China show the virus has become more genetically diverse Scientists in China have identified an influenza virus that they say has the potential to spread around the world, sickening and killing people whose immune systems ... […]

  • H7N9 bird flu resurges in China ahead of Lunar New Year
    on January 27, 2014 at 9:04 pm

    He was diagnosed as having H7N9 virus, a new strain of avian flu that jumped from birds to humans for the first time last year, said his close cousin, who requested not to be identified. "I couldn't believe it," she said. "How could this happen to him? […]

via Google News and Bing News

Other Interesting Posts

Leave a Reply

%d bloggers like this: