Russian scientists at the Moscow Institute of Physics and Technology (MIPT), the Joint Institute for High Temperatures of the Russian Academy of Sciences (JIHT RAS), and Gamaleya Research Centre of Epidemiology and Microbiology found that treating cells with cold plasma leads to their regeneration and rejuvenation. This result can be used to develop a plasma therapy program for patients with non-healing wounds.
The paper has been published in the Journal of Physics D: Applied Physics.
Non-healing wounds make it more difficult to provide effective treatment to patients and are therefore a serious problem faced by doctors. These wounds can be caused by damage to blood vessels in the case of diabetes, failure of the immune system resulting from an HIV infection or cancers, or slow cell division in elderly people. Treatment of non-healing wounds by conventional methods is very difficult and in some cases impossible.
Cold atmospheric-pressure plasma refers to a partially ionized gas (the proportion of charged particles in the gas being close to 1?%) with a temperature below 100,000 K. Its application in biology and medicine has been made possible by the advent of plasma sources generating jets at 30–40?°C.
An earlier study established the bactericidal properties of low-temperature plasma, as well as the relatively high resistance of cells and tissues to its influence. The results of plasma treatment of patients with non-healing wounds varied from positive to neutral. The authors’ previous work prompted them to investigate the possibility that the effect of plasma treatment on wound healing could depend on application pattern (the interval between applications and the total number of applications).
Two types of cells were used in this study, viz. fibroblasts (connective tissue cells) and keratinocytes (epithelial cells). Both play a central role in wound healing.
The effect of plasma treatment on cells was measured. In fibroblast samples, the number of cells increased by 42.6?% after one application (A) and by 32.0?% after two applications (B), as compared to the untreated controls. While no signs of DNA breaks were detected following plasma application, an accumulation of cells in the active phases of the cell cycle was observed, alongside a prolonged growth phase (30 hours). This means that the effect of plasma could be characterized as regenerative, as opposed to harmful.
The proliferation of cells that had been treated daily over a period of three days (group C) was reduced by 29.1?% relative to the controls. Keratinocytes did not show noticeable changes in proliferation.
The researchers also performed an assay of the senescence-associated ?-galactosidase, which is measured at pH 6.0. The concentration of this enzyme in a cell increases with age. Plasma treatment significantly reduced the content of this substance in the samples. This, together with a prolonged exponential growth phase of the culture, suggests a functional activation of cells—their rejuvenation.
‘The positive response to plasma treatment that we observed could be linked to the activation of a natural destructive mechanism called autophagy, which removes damaged organelles from the cell and reactivates cellular metabolic processes,’ says Elena Petersen, a co-author of the paper and the head of the Laboratory of Cellular and Molecular Technologies at MIPT.
The scientists are planning additional research into the molecular mechanisms underlying the effects of plasma on cells. They also aim to determine the influence of a patient’s age on the effectiveness of plasma therapy.
Learn more: Cold plasma will heal non-healing wounds
The Latest on: Non-healing wounds
via Google News
The Latest on: Non-healing wounds
- Healogics Newest Service Line Drives Competitive Advantage with Data on December 18, 2018 at 7:46 am
"With our nationwide network of nearly 700 Wound Care Centers®, we care for more than 330,000 patients living with non-healing wounds each year," said David Bassin, Chief Executive Officer at ... […]
- BMC wound center re-accredited on December 15, 2018 at 9:35 pm
The Center for Wound Care and Hyperbaric Medicine offers a comprehensive approach for treating patients with non-healing wounds. It is staffed with specially trained physicians, nurses and technicians ... […]
- Healogics Wound Science Initiative Addresses Social Determinants of Health on December 6, 2018 at 7:27 am
Healogics is focused on improving the care of people living with chronic, non-healing wounds. The Wound Science Initiative, launched in 2017, strives to advance the science, awareness and practice ... […]
- Wound Care Center marks two years on December 5, 2018 at 11:56 am
“Our clinic is equipped with state-of-the-art treatments such as wound debridement, specialized dressing selections, compression wraps, skin substitutes, hyperbaric oxygen therapy and patient educatio... […]
- Wound Closure Products Market is Expected to Surpass CAGR of 6.3% During 2015 - 2023 on December 5, 2018 at 11:27 am
According to an article published in Wound Repair and Regeneration in 2009, over 2% of the population of the U.S. suffers from chronic non-healing wounds. Statistics published in the Journal of Wound ... […]
- Gaza: Thousands of complex gunshot wounds require urgent treatment on November 29, 2018 at 1:56 pm
"If it goes untreated, it can lead to non-healing wounds and increase the risk of amputation. And as time goes on, it gets worse. These infections need to be treated as soon as possible. It's terrifyi... […]
- How, When, and Why Honey Is Used for Wound Care on November 28, 2018 at 6:25 am
If you aren’t seeing any improvement, talk to a doctor or nurse. Medical grade honey on wounds has been shown to help people with chronic and non-healing wounds. Medical honey has anti-bacterial, anti ... […]
- Researchers develop new method to diagnose, monitor chronic wounds on November 20, 2018 at 7:08 pm
Chronic skin wounds affect more than 150 million people worldwide and cost approximately $25 billion in health care annually in the United States alone. These non-healing wounds are characterized by i... […]
- Healogics Unveils Patient Engagement Program on November 7, 2018 at 12:44 pm
The Patient Engagement program will allow the company to extend their reach and connect more directly with people who are living with ostomies and chronic, non-healing wounds. […]
via Bing News