A tool that provides world-class microscopy and spatially resolved chemical analysis shows considerable promise for advancing a number of areas of study, including chemical science, pharmaceutical development and disease progression.
The hybrid optical microscope/mass spectrometry-based imaging system developed at the Department of Energy’s Oak Ridge National Laboratory operates under ambient conditions and requires no pretreatment of samples to analyze chemical compounds with sub-micron resolution. One micron is equal to about 1/100th the width of a human hair. Results of the work by postdoctoral associate Jack Cahill and Gary Van Berkel and Vilmos Kertesz of ORNL’s Chemical Sciences Division are detailed in Analytical Chemistry.
“Knowing the chemical basis of material interactions that take place at interfaces is vital for designing and advancing new functional materials that are important for DOE missions such as organic photovoltaics for solar energy,” Van Berkel said. “In addition, the new tool can be used to better understand the chemical basis of important biological processes such as drug transport, disease progression and response for treatment.”
The hybrid instrument transfers tiny amounts of a material such as human tissue or an organic polymer from a sample by a laser ablation process in which material is captured and transported via liquid stream to the ionization source of the mass spectrometer. In just seconds, a computer screen displays the results.
Researchers noted that the resolution of less than one micron is essential to accurately differentiate and distinguish between polymers and sub-components of similar-sized cells.
“Today’s mass spectrometry imaging techniques are not yet up to the task of reliably acquiring molecular information on a wide range of compound types,” Cahill said. “Examples include synthetic polymers used in various functional materials like light harvesting and emitting devices or biopolymers like cellulose in plants or proteins in animal tissue.”
This technology, however, provides the long-sought detailed chemical analysis through a simple interface between a hybrid optical microscope and an electrospray ionization system for mass spectrometry.
The Latest on: Microscopy
via Google News
The Latest on: Microscopy
- High Resolution 3D X-Ray Microscopy Market by Application and Type Forecasts 2019-2026: Nikon, Carl Zeiss Microscopy, Phenom-World, Matsusada on April 17, 2019 at 2:27 am
Data on High Resolution 3D X-Ray Microscopy core competencies to the prominent market players and market opportunities are presented through self –explanatory charts, tables and graphic images. This ... […]
- Transmission Electron Microscopy (TEM) Market Segmentation, Parameters and Prospects 2019 to 2025 Market Research Report on April 17, 2019 at 1:02 am
New Study On '2019-2025 Transmission Electron Microscopy (TEM) Market Global Key Player, Demand, Growth, Opportunities and Analysis Forecast Added to Wise Guy Reports Database The global ... […]
- New Microscopy Technique Measures Activity of Deep Brain Regions on April 16, 2019 at 7:30 am
Since the 1990’s, two-photon (2p) scanning microscopy has been the standard for brain imaging. Now, a novel microscopy technique developed by a team at the Rockefeller University, called hybrid ... […]
- Microscopy in the body on April 16, 2019 at 7:10 am
Biotechnologists, physicists, and medical researchers at Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU) have developed technology for microscopic imaging in living organisms. A miniaturised ... […]
- Quantum Effects In Imaging Nano-Structures Using Photon-Induced Near-Field Electron Microscopy on April 16, 2019 at 2:22 am
In this paper, we introduce the quantum mechanical approach as a more physically-realistic model to accurately quantify the electron-photon interaction in Photon-induced near-field electron microscopy ... […]
- Rockefeller scientists develop novel microscopy technique that peers deep into the brain on April 16, 2019 at 12:51 am
In order to understand the brain, scientists must be able to see the brain--cell by cell, and moment by moment. However, because brains comprise billions of microscopic moving parts, faithfully ... […]
- Interferometric scattering microscopy reveals microsecond nanoscopic protein motion on a live cell membrane on April 15, 2019 at 11:43 am
Many of the biological functions of a cell are dictated by the intricate motion of proteins within its membrane over a spatial range of nanometres to tens of micrometres and time intervals of ... […]
- New microscopy method promises better picture of deep brain activity on April 12, 2019 at 8:51 am
April 12 (UPI) --Scientists expect a new microscopy technique to offer a more comprehensive picture of deep brain activity. Imaging the brain is hard. Not only is it composed of millions of neurons ... […]
- New microscopy technique peers deep into the brain on April 12, 2019 at 6:16 am
In order to understand the brain, scientists must be able to see the brain—cell by cell, and moment by moment. However, because brains comprise billions of microscopic moving parts, faithfully ... […]
- Analysis of bright spot in confocal microscopy images to diagnose brain tumors on April 11, 2019 at 10:20 pm
Photodynamic diagnosis using 5-aminolevulinic acid (5-ALA) is now widely used for neurosurgical resection of brain tumors. Distinguishing a tumor from healthy tissue is based on greater 5-ALA-derived ... […]
via Bing News