Imaging technique tells tumor tissue from normal tissue, could be used in operating room for real-time guidance of surgery
A new laser-based technology may make brain tumor surgery much more accurate, allowing surgeons to tell cancer tissue from normal brain at the microscopic level while they are operating, and avoid leaving behind cells that could spawn a new tumor.
In a new paper, featured on the coverof the journal Science Translational Medicine, a team of University of Michigan Medical School andHarvard University researchers describes how the technique allows them to “see” the tiniest areas of tumor cells in brain tissue.
They used this technique to distinguish tumor from healthy tissue in the brains of living mice — and then showed that the same was possible in tissue removed from a patient with glioblastoma multiforme, one of the most deadly brain tumors.
Now, the team is working to develop the approach, called SRS microscopy, for use during an operation to guide them in removing tissue, and test it in a clinical trial at U-M. The work was funded by the National Institutes of Health.
A need for improvement in tumor removal
On average, patients diagnosed with glioblastoma multiforme live only 18 months after diagnosis. Surgery is one of the most effective treatments for such tumors, but less than a quarter of patients’ operations achieve the best possible results, according to a study published last fall in the Journal of Neurosurgery.
“Though brain tumor surgery has advanced in many ways, survival for many patients is still poor, in part because surgeons can’t be sure that they’ve removed all tumor tissue before the operation is over,” says co-lead author Daniel Orringer, M.D., a lecturer in the U-M Department of Neurosurgery who has worked with the Harvard team since a chance meeting with a team member during his U-M residency.
“We need better tools for visualizing tumor during surgery, and SRS microscopy is highly promising,” he continues. “With SRS we can see something that’s invisible through conventional surgical microscopy.”
The Latest on: SRS microscopy
Developments in Brain Studies Using Raman Spectroscopy - AZoM
June 7, 2018 - To optimize the use of this technique, coherent Raman spectroscopy (CRS) microscopy is used in tandem to increase the intensity of the generated signals, using coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS).
April 3, 2018 - Stimulated Raman scattering (SRS) microscopy, based on the coherent interaction of 2 different laser beams with vibrational levels in the molecules of the sample, would enable much faster detection and identification of microplastics. Here, we present ...
Raman imaging identifies which bacteria are susceptible to antibiotics - Chemical & Engineering News
March 19, 2018 - To accelerate the process, Mohamed N. Seleem of Purdue University, Ji-Xin Cheng of Boston University, and coworkers used stimulated Raman scattering (SRS) microscopy of carbon-deuterium bonds to monitor glucose metabolism of Enterococcus faecalis ...
How saturated fatty acids damage cells - Science Daily
December 1, 2017 - By making the switch, all molecules made from fatty acids can be observed inside living cells by an advanced imaging technique called stimulated Raman scattering (SRS) microscopy. What the researchers found using this technique could have significant ...
Expanding Bioimaging's Palette - Optics & Photonics News (blog)
April 26, 2017 - Team leader Wei Min says the detection sensitivity of the group's epr-SRS microscopy platform is several orders of magnitude better than conventional Raman microscopy because it “harnesses the stimulated emission process to amplify the otherwise weak ...
April 25, 2017 - IMAGE: A new microscopy technique is able to image more biomolecules than traditional methods limited to five fluorescent markers at a time, improving biological disease studies. (Image credit: Columbia University). Columbia University (New York, NY) ...
New microscopy method breaks color barrier of optical imaging - Science Daily
April 20, 2017 - Researchers at Columbia University developed a new optical microscopy platform called electronic pre-resonance stimulated Raman scattering (epr-SRS) microscopy that combines a high level of sensitivity and selectivity. The innovative technique allows ...
New Spectroscopy Method Detects Myelin Changes in Early ALS - Alzforum
November 12, 2016 - In SRS microscopy, two coordinated laser beams are aimed at a sample that absorbs photons and then re-emits them, albeit with slightly different energies depending on the structure of the molecules present. In effect, each molecule creates its own ...
Stimulated Raman Scattering (SRS) microscopy and cancer detection - News-Medical.net
November 24, 2015 - Images collected using SRS microscopy show that normal brain tissue has sparse cells that contain bundles of axons, whereas cancerous tissue is full of cells organized in a disordered fashion. SRS microscopy has the potential to be used in a wide range ...
-
Developments in Brain Studies Using Raman Spectroscopy
on June 7, 2018 at 5:05 am
To optimize the use of this technique, coherent Raman spectroscopy (CRS) microscopy is used in tandem to increase the intensity of the generated signals, using coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS). These ... […]
-
New microscopy method breaks color barrier of optical imaging
on April 18, 2017 at 5:00 pm
Researchers at Columbia University developed a new optical microscopy platform called electronic pre-resonance stimulated Raman scattering (epr-SRS ... step toward breaking the so-called "color barrier" of light microscopy for biological systems, allowing ... […]
-
Laser-based imaging tool could increase accuracy, safety of brain tumor surgery
on October 15, 2015 at 9:33 am
Images collected using an SRS microscope show that normal brain contains sparse cells with bundles of nerve fibers, called axons (left), but brain tumor tissue is full of cells in a disordered pattern (right). While you can see the difference on a ... […]
-
New laser-based tool could dramatically improve the accuracy of brain tumor surgery
on September 4, 2013 at 11:21 am
This image of a human glioblastoma brain tumor in the brain of a mouse was made with stimulated Raman scattering, or SRS, microscopy. The technique allows the tumor (blue) to be easily distinguished from normal tissue (green) based on faint signals emitted ... […]
-
Video microscopy reveals molecules in motion
on December 6, 2010 at 4:00 pm
Called stimulated Raman scattering (SRS) microscopy, the technique is the culmination of more than a decade of work in Sunney Xie's chemistry lab at Harvard University in Cambridge, Massachusetts. The advance is reported today in the journal Science 1. […]
-
Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering
on December 2, 2010 at 4:00 pm
Stimulated Raman scattering (SRS) microscopy allows highly sensitive optical imaging based on vibrational spectroscopy without adding toxic or perturbative labels. However, SRS imaging in living animals and humans has not been feasible because light cannot ... […]
-
New microscopy tracks molecules in live tissue at video rate
on December 2, 2010 at 6:43 am
For the first time, SRS microscopy makes possible label-free chemical movies, with streaming footage at the subcellular level, catching video of proteins, lipids, and water within cells. "When we started this project 11 years ago, we never imagined we'd ... […]
-
New microscopy tracks molecules in live tissue at video rate
on December 1, 2010 at 4:00 pm
For the first time, SRS microscopy makes possible label-free chemical movies, with streaming footage at the subcellular level, catching video of proteins, lipids, and water within cells. "When we started this project 11 years ago, we never imagined we'd ... […]
-
Microscopic marvels: The naked microscope
on June 2, 2009 at 5:00 pm
Then in December last year, Freudiger together with postdoc Wei Min reported a further improvement 1. Called stimulated Raman scattering microscopy (SRS), the method excites molecules with two laser beams that have been calibrated so that the difference ... […]
-
Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy
on December 18, 2008 at 4:00 pm
1 Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA. 2 Department of Physics, Harvard University, Cambridge, MA 02138, USA. 3 Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston ... […]
via Google News and Bing News
