A group of stem-cell biologists have grown an “organoid” that resembles a brain
REGENERATIVE medicine, the science of producing tissues and organs from stem cells, is a rapidly developing field.
This week, however, it took a leap forward that was big even by its own demanding standards. A team of researchers led by Madeline Lancaster of the Austrian Academy of Sciences, in Vienna, announced that they have grown things which, while not human brains, resemble brains in important ways.
Dr Lancaster’s organoids, as she calls them, are a far cry from the brains in jars beloved of the writers of horror movies. But they do contain several recognisably different types of nerve cell and have anatomical features which look like those of real brains. They might be used to study, in ways that would be unethical in a living human being or impossible even in a mouse, the crucial early stages of brain development, and how they can go wrong. They could be employed to test drugs in ways that mere cell cultures cannot be. And because they can be made, if needed, from the cells of living people, they might even illuminate the particular problems of individual patients.
To make an organoid, Dr Lancaster’s team start, as they describe in an article in Nature, with what is known as an embryoid body. Just as an organoid has some features of an organ without truly being one, so an embryoid body has some features of an embryo without actually being one. Embryoid bodies can be grown either from natural stem cells—themselves derived ultimately from real embryos—or from what are known as induced pluripotent cells, which are made from adult cells (usually skin cells) that have been treated with four crucial biochemical factors which cause them to forget their identity and behave like embryonic cells.
Embryos have three layers: endoderm, mesoderm and ectoderm. Each turns into an eclectic mixture of body parts in the complete organism. Nervous systems grow from the ectoderm (which also contributes dental enamel and the skin’s epidermis, among other things), so the team put ectodermal cells into droplets of gel and then floated the droplets in a nutrient broth in a gently rotating bioreactor (which allowed the cells to grow without being shaped by the constraint of a vessel such as a Petri dish) to see what would happen.
Though the result (pictured above) may not look much like a brain to a layman, to an expert the resemblance is remarkable. After ten days the organoid has developed neurons. After 30 days it has regions recognisably similar to some of those in a real brain. And though, because they lack the blood supply of a real brain, organoids never grow much bigger than 4mm across, they live a long time. Some are now a year old and still going strong.
Real brains consist in large measure of layers of neurons called the cortex. This surrounds fluid-filled spaces known as ventricles. That is more or less the anatomy of an organoid. Many of them also contain areas which look like choroid plexuses. These are places that absorb nutrients from the bloodstream and dump waste into it. They also generate the cerebrospinal fluid that fills ventricles.
Signs of other structures turn up too. The various lobes of a real brain sport different mixtures of neurons. The team see signs of this in the organoids. They found evidence of retinas (the back of the eye is an outgrowth of the brain), of meninges (the membranes that surround the brain) and of hippocampal cells (the hippocampus is a part of the brain which is crucial for memory formation). The organoids, then, look as though they are making a fair fist of trying to become real brains.
So the method clearly works.
The Latest on: Organoids
- Human placenta organoids grown outside womb on December 3, 2018 at 7:50 am
Scientists have produced elaborate placenta-like organoids in a dish, which may aid in the research of pregnancy disorders. The results, published in the journal Nature, reveal that these organoids, c... […]
- Brain, muscle cells found lurking in kidney organoids grown in lab on December 1, 2018 at 3:16 pm
Newswise — Scientists hoping to develop better treatments for kidney disease have turned their attention to growing clusters of kidney cells in the lab. One day, so-called organoids – grown ... […]
- expert reaction to placental organoids being created in vitro on November 29, 2018 at 2:03 am
Research published in Nature demonstrates the creation of cell cultures of trophoblast – the unique cell type of the placenta. Prof Magdalena Zernicka-Goetz, Professor of Mammalian Development and Ste... […]
- Long-Lasting Placenta Organoids Could Improve Pregnancy Research on November 29, 2018 at 2:02 am
Researchers have made 3D placenta organoids from human cells that can last for over a year, improving over the current models to study disease in pregnancy and develop drugs. Researchers at the Univer... […]
- “Placental organoids” could be the future of pregnancy and conception research say researchers on November 28, 2018 at 10:07 pm
Researchers have successfully managed to develop “mini placentas” or placenta organoids in the laboratories that could transform research of pregnancy, conception, stillbirth, miscarriages and pregnan... […]
- Medical science: Functional placenta organoids developed on November 28, 2018 at 6:39 pm
The creation of stable, long-term, human placenta organoids is described in a paper published online in Nature this week. These organoid models represent a significant innovation for the study of huma... […]
- Scientists create 'mini placenta' organoids in a lab dish on November 28, 2018 at 10:51 am
LONDON (Reuters) - Scientists in Britain have succeeded in creating mini human placenta organoids which they say will transform scientific understanding of reproductive disorders such as pre ... […]
- Preventing the Wrong Cells From Forming in Organoids on November 21, 2018 at 7:48 am
Researchers have long relied on models to study biology in the lab. Cells are a commonly used model; they are often grown in a layer in a dish or liquid suspension in a flask. But that is not how cell... […]
- Fresh urgency in mapping out ethics of brain organoid research on November 20, 2018 at 5:05 pm
University of Oxford provides funding as a member of The Conversation UK. Scientists have become increasingly adept at creating brain organoids – which are essentially miniature human brains grown in ... […]
- For Difficult-to-Model Brain Diseases, Brain Organoids Come to the Rescue on November 19, 2018 at 4:00 pm
Madeline Lancaster, PhD, started her postdoctoral work in 2010 with plans to investigate the basic mechanisms of neurogensis using 2-dimensional (2D) cell culture methods. But a happy accident set her ... […]
via Google News and Bing News